LncRNA HCG18 promotes cell multiplication and metastasis by miR-148b/ETV5 regulation in osteosarcoma

Zhipan Zheng; Kai Lin

LncRNA HCG18 promotes cell multiplication and metastasis by miR-148b/ETV5 regulation in osteosarcoma

Am J Transl Res. 2021 Jul 15;13(7):7783-7793. eCollection 2021.

Authors

Zhipan Zheng¹, Kai Lin²

Affiliations

¹ Department of Trauma Surgery, Hubei Hanchuan People's Hospital Hanchuan, People's Republic of China.
² Department of Interventional Vascular Surgery, Hubei Hanchuan People's Hospital Hanchuan, People's Republic of China.

PMID: 34377255
PMCID: PMC8340218

Abstract

Objective: To determine the effects of long non-coding RNA (LncRNA) HCG18 on cell multiplication and invasion of osteosarcoma.

Methods: MTT assay and transwell assay were used for cell multiplication and invasion, respectively. Real-time quantitative polymerase chain reaction (RT-qPCR) and western blot were used to determine transcriptional and translational expression. Luciferase reporter assay was used to identify the specific target relationships.

Results: The expression of LncRNA HCG18 was dramatically increased in osteosarcoma cells compared to the normal tissues. LncRNA-HCG18 accelerated cell multiplication and invasion in vitro, which was achieved by down-regulating the overexpression of miR-148b, and down-regulating ETV5, indicating combination of ETV5 and miR-148b in osteosarcoma. Overexpression of ETV5 could reverse the inhibitory effect of knockout of lncRNA HCG18 on cell multiplication and invasion.

Conclusion: LncRNA HCG18 acted as a sponge of miR-148b and played an oncogenic role in osteosarcoma, providing therapeutic targets for osteosarcoma.

Keywords: LncRNA HCG18; cell multiplication; invasion; osteosarcoma.