Protocol for setup and circadian analysis of inverted feeding in mice

STAR Protoc. 2021 Jul 28;2(3):100701. doi: 10.1016/j.xpro.2021.100701. eCollection 2021 Sep 17.

Abstract

Inverted feeding is a paradigm to study synchronization of circadian clocks by feeding rhythm in tissues more directly. Here, we provide a protocol for performing inverted feeding in mice and analyzing circadian rhythmicity in mouse tissues. We describe setting up inverted feeding and performing tissue dissection, followed by RNA extraction and gene expression analysis, and lastly R software-based analysis of circadian rhythmicity. This protocol can be combined with the use of CircaMetDB database for mechanistic studies of inverted feeding. For complete details on the use and execution of this protocol, please refer to Xin et al. (2021).

Keywords: Behavior; Bioinformatics; Gene Expression; Metabolism; Model Organisms; Molecular Biology; Systems biology.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Circadian Clocks / genetics
  • Circadian Rhythm / genetics
  • Circadian Rhythm / physiology*
  • Computational Biology / methods*
  • Feeding Behavior / physiology
  • Metabolism / physiology
  • Mice
  • Period Circadian Proteins / genetics

Substances

  • Period Circadian Proteins