Decreased convulsive threshold and memory loss after anti-NMDAR positive CSF injection in zebrafish

J Neuroimmunol. 2021 Oct 15;359:577689. doi: 10.1016/j.jneuroim.2021.577689. Epub 2021 Aug 8.


Anti-N-methyl-d-aspartate receptor (anti-NMDAR) encephalitis initially promotes memory deficits, behavioral changes, and epileptic seizures. We developed a new animal model of anti-NMDAR encephalitis using a single cerebroventricular injection of CSF from patients in adult zebrafish. We observed a reduction of the seizure threshold and recent memory deficits in those animals injected with CSF from patients with anti-NMDAR encephalitis. The locomotor activity was similar in the CSF and control groups. This zebrafish model consistently recapitulates symptoms seen in patients with anti-NMDAR encephalitis. It may provide a reliable, fast and cost-effective platform to investigate new therapeutic strategies to anti-NMDAR encephalitis.

Keywords: Encephalitis; Experimental model; Memory; Nmda; Receptor; Seizure; Zebrafish.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-N-Methyl-D-Aspartate Receptor Encephalitis / cerebrospinal fluid*
  • Anti-N-Methyl-D-Aspartate Receptor Encephalitis / immunology*
  • Cerebrospinal Fluid / immunology*
  • Female
  • Humans
  • Injections, Intraventricular
  • Locomotion / immunology
  • Male
  • Memory Disorders / immunology*
  • Memory Disorders / prevention & control
  • Seizures / immunology*
  • Seizures / prevention & control
  • Zebrafish