Tissue specificity of DNA repair: the CRISPR compass

Joana Ferreira da Silva; Mathilde Meyenberg; Joanna I Loizou

doi:10.1016/j.tig.2021.07.010

Tissue specificity of DNA repair: the CRISPR compass

Trends Genet. 2021 Nov;37(11):958-962. doi: 10.1016/j.tig.2021.07.010. Epub 2021 Aug 12.

Authors

Joana Ferreira da Silva¹, Mathilde Meyenberg¹, Joanna I Loizou²

Affiliations

¹ CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, 1090 Vienna, Austria; Institute of Cancer Research, Department of Medicine I, Comprehensive Cancer Center, Medical University of Vienna, 1090 Vienna, Austria.
² CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, 1090 Vienna, Austria; Institute of Cancer Research, Department of Medicine I, Comprehensive Cancer Center, Medical University of Vienna, 1090 Vienna, Austria. Electronic address: joanna.loizou@meduniwien.ac.at.

PMID: 34392967
DOI: 10.1016/j.tig.2021.07.010

Abstract

CRISPR-Cas9-mediated genome editing holds great promise for the correction of pathogenic variants in humans. However, its therapeutic implementation is hampered due to unwanted editing outcomes. A better understanding of cell type- and tissue-specific DNA repair processes will ultimately enable precise control of editing outcomes for safer and effective therapies.

Keywords: CRISPR-Cas9; genome editing; progenitor cells; stem cells; tissue-specific DNA repair.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

CRISPR-Cas Systems* / genetics
DNA Repair / genetics
Gene Editing*
Humans
Organ Specificity / genetics