Single-Cell RNA Sequencing of Retina:New Looks for Gene Marker and Old Diseases

doi:10.3389/fmolb.2021.699906

Review

. 2021 Jul 30:8:699906.

doi: 10.3389/fmolb.2021.699906. eCollection 2021.

Single-Cell RNA Sequencing of Retina:New Looks for Gene Marker and Old Diseases

Peixi Ying¹, Chang Huang^{2

3

4

5}, Yan Wang⁶, Xi Guo⁷, Yuchen Cao¹, Yuxi Zhang¹, Sheng Fu⁸, Lin Chen⁹, Guoguo Yi¹⁰, Min Fu¹¹

Affiliations

¹ The Second Clinical School, Southern Medical University, Guangzhou, China.
² Eye Institute and Department of Ophthalmology, Eye & ENT Hospital, Fudan University, Shanghai, China.
³ NHC Key Laboratory of Myopia, Fudan University, Shanghai, China.
⁴ Key Laboratory of Myopia, Chinese Academy of Medical Sciences, Shanghai, China.
⁵ Shanghai Key Laboratory of Visual Impairment and Restoration, Shanghai, China.
⁶ Department of Ophthalmology, South China Hospital, Health Science Center, Shenzhen University, Shenzhen, China.
⁷ Medical College of Rehabiliation, Southern Medical University, Guangzhou, China.
⁸ The University of South China, Hengyang, China.
⁹ Department of Anesthesiology, Shenzhen Hospital, Southern Medical University, Shenzhen, China.
¹⁰ Department of Ophthalmology, the Sixth Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
¹¹ Department of Ophthalmology, Zhujiang Hospital, Southern Medical University, Guangzhou, China.

PMID: 34395530
PMCID: PMC8362665
DOI: 10.3389/fmolb.2021.699906

Review

Single-Cell RNA Sequencing of Retina:New Looks for Gene Marker and Old Diseases

Peixi Ying et al. Front Mol Biosci. 2021.

. 2021 Jul 30:8:699906.

doi: 10.3389/fmolb.2021.699906. eCollection 2021.

Authors

Peixi Ying¹, Chang Huang^{2

3

4

5}, Yan Wang⁶, Xi Guo⁷, Yuchen Cao¹, Yuxi Zhang¹, Sheng Fu⁸, Lin Chen⁹, Guoguo Yi¹⁰, Min Fu¹¹

Affiliations

¹ The Second Clinical School, Southern Medical University, Guangzhou, China.
² Eye Institute and Department of Ophthalmology, Eye & ENT Hospital, Fudan University, Shanghai, China.
³ NHC Key Laboratory of Myopia, Fudan University, Shanghai, China.
⁴ Key Laboratory of Myopia, Chinese Academy of Medical Sciences, Shanghai, China.
⁵ Shanghai Key Laboratory of Visual Impairment and Restoration, Shanghai, China.
⁶ Department of Ophthalmology, South China Hospital, Health Science Center, Shenzhen University, Shenzhen, China.
⁷ Medical College of Rehabiliation, Southern Medical University, Guangzhou, China.
⁸ The University of South China, Hengyang, China.
⁹ Department of Anesthesiology, Shenzhen Hospital, Southern Medical University, Shenzhen, China.
¹⁰ Department of Ophthalmology, the Sixth Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
¹¹ Department of Ophthalmology, Zhujiang Hospital, Southern Medical University, Guangzhou, China.

PMID: 34395530
PMCID: PMC8362665
DOI: 10.3389/fmolb.2021.699906

Abstract

The retina is composed of 11 types of cells, including neurons, glial cells and vascular bed cells. It contains five types of neurons, each with specific physiological, morphological, and molecular definitions. Currently, single-cell RNA sequencing (sRNA-seq) is emerging as one of the most powerful tools to reveal the complexity of the retina. The continuous discovery of retina-related gene targets plays an important role in helping us understand the nature of diseases. The revelation of new cell subpopulations can focus the occurrence and development of diseases on specific biological activities of specific cells. In addition, sRNA-seq performs high-throughput sequencing analysis of epigenetics, transcriptome and genome at the single-cell level, with the advantages of high-throughput and high-resolution. In this paper, we systematically review the development history of sRNA-seq technology, and summarize the new subtypes of retinal cells and some specific gene markers discovered by this technology. The progress in the diagnosis of retinal related diseases is also discussed.

Keywords: ScRNA-seq; gene; retina; retinal disease; single-cell RNA sequencing.

PubMed Disclaimer

Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

**FIGURE 1**
Summary of the development of Single-cell RNA sequecing technology.

See this image and copyright information in PMC

Cited by

Single cell transcriptome analyses of the developing zebrafish eye- perspectives and applications.
Vöcking O, Famulski JK. Vöcking O, et al. Front Cell Dev Biol. 2023 Jun 29;11:1213382. doi: 10.3389/fcell.2023.1213382. eCollection 2023. Front Cell Dev Biol. 2023. PMID: 37457291 Free PMC article. Review.
Single-cell transcriptomics of the ocular anterior segment: a comprehensive review.
Ahsanuddin S, Wu AY. Ahsanuddin S, et al. Eye (Lond). 2023 Nov;37(16):3334-3350. doi: 10.1038/s41433-023-02539-3. Epub 2023 May 3. Eye (Lond). 2023. PMID: 37138096 Free PMC article. Review.
Definition of the transcriptional units of inherited retinal disease genes by meta-analysis of human retinal transcriptome data.
Ruiz-Ceja KA, Capasso D, Pinelli M, Del Prete E, Carrella D, di Bernardo D, Banfi S. Ruiz-Ceja KA, et al. BMC Genomics. 2023 Apr 18;24(1):206. doi: 10.1186/s12864-023-09300-w. BMC Genomics. 2023. PMID: 37072692 Free PMC article.
Single cell RNA-seq analysis reveals temporally-regulated and quiescence-regulated gene expression in Drosophila larval neuroblasts.
Dillon N, Cocanougher B, Sood C, Yuan X, Kohn AB, Moroz LL, Siegrist SE, Zlatic M, Doe CQ. Dillon N, et al. Neural Dev. 2022 Aug 24;17(1):7. doi: 10.1186/s13064-022-00163-7. Neural Dev. 2022. PMID: 36002894 Free PMC article.
Identification of Genetic Predisposition in Noncirrhotic Portal Hypertension Patients With Multiple Renal Cysts by Integrated Analysis of Whole-Genome and Single-Cell RNA Sequencing.
Wu Y, Wu Y, Liu K, Liu H, Wang S, Huang J, Ding H. Wu Y, et al. Front Genet. 2021 Nov 12;12:775470. doi: 10.3389/fgene.2021.775470. eCollection 2021. Front Genet. 2021. PMID: 34868264 Free PMC article.

References

1. Anguita R., Tasiopoulou A., Shahid S., Roth J., Sim S. Y., Patel P. J. (2021). A Review of Aflibercept Treatment for Macular Disease[J]. Ophthalmol. Ther. 10.1007/s40123-021-00354-1 - DOI - PMC - PubMed
1. Author Anonymous (2020). Method of the Year 2019: Single-Cell Multimodal Omics. Nat. Methods 17 (1), 1. 10.1038/s41592-019-0703-5 - DOI - PubMed
1. Baden T., Berens P., Franke K., Román Rosón M., Bethge M., Euler T. (2016). The Functional Diversity of Retinal Ganglion Cells in the Mouse. Nature 529 (7586), 345–350. 10.1038/nature16468 - DOI - PMC - PubMed
1. Banin E., Gootwine E., Obolensky A., Ezra-Elia R., Ejzenberg A., Zelinger L., et al. (2015). Gene Augmentation Therapy Restores Retinal Function and Visual Behavior in a Sheep Model of CNGA3 Achromatopsia. Mol. Ther. 23 (9), 1423–1433. 10.1038/mt.2015.114 - DOI - PMC - PubMed
1. Benowitz L. I., He Z., Goldberg J. L. (2017). Reaching the Brain: Advances in Optic Nerve Regeneration. Exp. Neurol. 287 (3), 365–373. 10.1016/j.expneurol.2015.12.015 - DOI - PubMed

Publication types

Actions

LinkOut - more resources

Full Text Sources

[1] Anguita R., Tasiopoulou A., Shahid S., Roth J., Sim S. Y., Patel P. J. (2021). A Review of Aflibercept Treatment for Macular Disease[J]. Ophthalmol. Ther. 10.1007/s40123-021-00354-1 - DOI - PMC - PubMed

[2] Anguita R., Tasiopoulou A., Shahid S., Roth J., Sim S. Y., Patel P. J. (2021). A Review of Aflibercept Treatment for Macular Disease[J]. Ophthalmol. Ther. 10.1007/s40123-021-00354-1 - DOI - PMC - PubMed

[3] Author Anonymous (2020). Method of the Year 2019: Single-Cell Multimodal Omics. Nat. Methods 17 (1), 1. 10.1038/s41592-019-0703-5 - DOI - PubMed

[4] Author Anonymous (2020). Method of the Year 2019: Single-Cell Multimodal Omics. Nat. Methods 17 (1), 1. 10.1038/s41592-019-0703-5 - DOI - PubMed

[5] Baden T., Berens P., Franke K., Román Rosón M., Bethge M., Euler T. (2016). The Functional Diversity of Retinal Ganglion Cells in the Mouse. Nature 529 (7586), 345–350. 10.1038/nature16468 - DOI - PMC - PubMed

[6] Baden T., Berens P., Franke K., Román Rosón M., Bethge M., Euler T. (2016). The Functional Diversity of Retinal Ganglion Cells in the Mouse. Nature 529 (7586), 345–350. 10.1038/nature16468 - DOI - PMC - PubMed

[7] Banin E., Gootwine E., Obolensky A., Ezra-Elia R., Ejzenberg A., Zelinger L., et al. (2015). Gene Augmentation Therapy Restores Retinal Function and Visual Behavior in a Sheep Model of CNGA3 Achromatopsia. Mol. Ther. 23 (9), 1423–1433. 10.1038/mt.2015.114 - DOI - PMC - PubMed

[8] Banin E., Gootwine E., Obolensky A., Ezra-Elia R., Ejzenberg A., Zelinger L., et al. (2015). Gene Augmentation Therapy Restores Retinal Function and Visual Behavior in a Sheep Model of CNGA3 Achromatopsia. Mol. Ther. 23 (9), 1423–1433. 10.1038/mt.2015.114 - DOI - PMC - PubMed

[9] Benowitz L. I., He Z., Goldberg J. L. (2017). Reaching the Brain: Advances in Optic Nerve Regeneration. Exp. Neurol. 287 (3), 365–373. 10.1016/j.expneurol.2015.12.015 - DOI - PubMed

[10] Benowitz L. I., He Z., Goldberg J. L. (2017). Reaching the Brain: Advances in Optic Nerve Regeneration. Exp. Neurol. 287 (3), 365–373. 10.1016/j.expneurol.2015.12.015 - DOI - PubMed

Save citation to file

Email citation

Add to Collections

Add to My Bibliography

Your saved search

Create a file for external citation management software

Your RSS Feed

Single-Cell RNA Sequencing of Retina:New Looks for Gene Marker and Old Diseases

Affiliations

Single-Cell RNA Sequencing of Retina:New Looks for Gene Marker and Old Diseases

Authors

Affiliations

Abstract

Conflict of interest statement

Figures

Similar articles

Cited by

References

Publication types

LinkOut - more resources

Full Text Sources

Abstract

Conflict of interest statement

Figures

Similar articles

Cited by

References

Publication types

Related information

LinkOut - more resources

Full Text Sources