In Vitro and In Vivo Activity of Oxazolidinone Candidate OTB-658 against Mycobacterium tuberculosis

Antimicrob Agents Chemother. 2021 Oct 18;65(11):e0097421. doi: 10.1128/AAC.00974-21. Epub 2021 Aug 16.

Abstract

In this work, we assess antituberculosis activity of OTB-658 in vitro and in vivo. In vitro, OTB-658 showed bacteriostatic effectiveness with a lower MIC than linezolid against Mycobacterium tuberculosis. The minimal bactericidal concentrations and time-kill curves for OTB-658 indicated inhibition activity similar to that of linezolid. OTB-658 entered macrophages to inhibit M. tuberculosis growth. OTB-658 had a low mutation frequency (10-8), which would prevent drug-resistant mutations from emerging in combination regimens. In vivo, OTB-658 reduced CFU counts in the lungs and slightly inhibited bacterial growth in the spleen in the early stage and steady state in acute and chronic murine TB models. These results support the preclinical evaluation of OTB-658 and further clinical trials in China.

Keywords: Mycobacterium tuberculosis; OTB-658; antituberculosis activity; oxazolidinone.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antitubercular Agents / pharmacology
  • Antitubercular Agents / therapeutic use
  • Linezolid / pharmacology
  • Mice
  • Microbial Sensitivity Tests
  • Mycobacterium tuberculosis*
  • Oxazolidinones* / pharmacology

Substances

  • Antitubercular Agents
  • Oxazolidinones
  • Linezolid