Antioxidant Activity-guided Phytochemical Investigation of Artemisia aucheri Boiss.: Isolation of Ethyl Caffeate and a Spinacetin Glycoside

Parvin Jahanbani; Sajjad Nasseri; Mahdi Mojarrab

doi:10.22037/ijpr.2019.15496.13140

Antioxidant Activity-guided Phytochemical Investigation of Artemisia aucheri Boiss.: Isolation of Ethyl Caffeate and a Spinacetin Glycoside

Iran J Pharm Res. 2021 Winter;20(1):82-90. doi: 10.22037/ijpr.2019.15496.13140.

Authors

Parvin Jahanbani¹, Sajjad Nasseri², Mahdi Mojarrab³

Affiliations

¹ Students Research Committee, Kermanshah University of Medical Science, Kermanshah, Iran.
² Department of Pharmacognosy and Pharmaceutical Biotechnology, School of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran.
³ Pharmaceutical Sciences Research Center, Health Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran.

Abstract

Antioxidant activity of five different extracts (petroleum ether, dichloromethane, ethyl acetate, ethanol and ethanol-water) of Artemisia aucheri aerial parts was investigated by three various methods: ferrous ion chelating (FIC) assay, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method and β-carotene bleaching (BCB) test. Total phenolic contents (TPC) were measured by Folin-Ciocalteu method. The hydroethanolic extract exhibited the stronger inhibitory activity in BCB and FIC assays than the other extracts. Among the extracts analyzed, the ethyl acetate and ethanolic extracts exhibited the highest TPC and DPPH radical scavenging activity, respectively. Reversed phase vacuum liquid chromatography of ethanolic extract (with the highest extraction yield) produced five fractions (A to E) which were subjected to all antecedent experiments. The same sample (Fraction C) showed the highest TPC and DPPH radical scavenging activity while there were no statistically significant correlations between TPC and EC₅₀ values of various antioxidant assays. Ethyl caffeate and a spinacetin glycoside were isolated from the most active fraction and their structures were established using spectroscopic analysis including NMR and MS.

Keywords: Artemisia aucheri; Ethyl caffeate; Ferrous ion chelating activity; Free radical scavenging activity; Spinacetin glycoside; Total phenolic content; Vacuum liquid chromatography.