Matrix-assisted laser desorption ionization mass spectrometry profiling of plasma exosomes evaluates osteosarcoma metastasis

Zhenzhen Han; Cheng Peng; Jia Yi; Yiwen Wang; Qi Liu; Yi Yang; Shuping Long; Liang Qiao; Yuhui Shen

doi:10.1016/j.isci.2021.102906

Matrix-assisted laser desorption ionization mass spectrometry profiling of plasma exosomes evaluates osteosarcoma metastasis

iScience. 2021 Jul 26;24(8):102906. doi: 10.1016/j.isci.2021.102906. eCollection 2021 Aug 20.

Authors

Zhenzhen Han^{1

2}, Cheng Peng¹, Jia Yi², Yiwen Wang², Qi Liu¹, Yi Yang², Shuping Long³, Liang Qiao², Yuhui Shen¹

Affiliations

¹ Department of Orthopedics, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200000, China.
² Department of Chemistry, Fudan University, Shanghai 200433, China.
³ Department of Clinical Laboratory Medicine, Shanghai Tenth People's Hospital of Tongji University, Shanghai, China.

Abstract

Osteosarcoma is the most common primary sarcoma of bone among adolescents, often characterized by early lung metastasis resulting in high mortality. Recently, exosomes have been used in liquid biopsy to monitor tumors. Herein, we used matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) to profile human plasma exosomes for the evaluation of osteosarcoma lung metastasis. Forty patients with osteosarcoma with (n = 20) or without (n = 20) lung metastasis as well as 12 heathy controls were recruited. Exosomes were isolated from human plasma for MALDI-TOF MS analysis. Multivariate statistical analyses were performed based on the MALDI-TOF mass spectra. The strategy can efficiently differentiate osteosarcomas from healthy controls and further discriminate osteosarcoma lung metastasis from non-lung metastasis. We identified seven exosomal proteins as potential biomarkers of osteosarcoma lung metastasis. The proposed method holds great promise to clinically diagnose osteosarcoma and monitor osteosarcoma lung metastasis.

Keywords: disease; genetics; microbiology.