POSTAR3: an updated platform for exploring post-transcriptional regulation coordinated by RNA-binding proteins

Nucleic Acids Res. 2022 Jan 7;50(D1):D287-D294. doi: 10.1093/nar/gkab702.


RNA-binding proteins (RBPs) play key roles in post-transcriptional regulation. Accurate identification of RBP binding sites in multiple cell lines and tissue types from diverse species is a fundamental endeavor towards understanding the regulatory mechanisms of RBPs under both physiological and pathological conditions. Our POSTAR annotation processes make use of publicly available large-scale CLIP-seq datasets and external functional genomic annotations to generate a comprehensive map of RBP binding sites and their association with other regulatory events as well as functional variants. Here, we present POSTAR3, an updated database with improvements in data collection, annotation infrastructure, and analysis that support the annotation of post-transcriptional regulation in multiple species including: we made a comprehensive update on the CLIP-seq and Ribo-seq datasets which cover more biological conditions, technologies, and species; we added RNA secondary structure profiling for RBP binding sites; we provided miRNA-mediated degradation events validated by degradome-seq; we included RBP binding sites at circRNA junction regions; we expanded the annotation of RBP binding sites, particularly using updated genomic variants and mutations associated with diseases. POSTAR3 is freely available at

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Arabidopsis / genetics
  • Arabidopsis / metabolism
  • Binding Sites
  • Cell Line
  • Databases, Genetic*
  • Datasets as Topic
  • Humans
  • Internet
  • MicroRNAs / classification
  • MicroRNAs / genetics*
  • MicroRNAs / metabolism
  • Molecular Sequence Annotation
  • Nucleic Acid Conformation
  • RNA Processing, Post-Transcriptional*
  • RNA, Circular / classification
  • RNA, Circular / genetics*
  • RNA, Circular / metabolism
  • RNA-Binding Proteins / classification
  • RNA-Binding Proteins / genetics*
  • RNA-Binding Proteins / metabolism
  • Sequence Analysis, RNA
  • Software*


  • MicroRNAs
  • RNA, Circular
  • RNA-Binding Proteins