[The composition of ascites lymphocytes in high-grade serous ovarian cancer and its correlation with clinical features]

Zhonghua Yi Xue Za Zhi. 2021 Aug 10;101(30):2375-2381. doi: 10.3760/cma.j.cn112137-20210211-00409.
[Article in Chinese]

Abstract

Objective: To explore the composition of ascites lymphocytes in high-grade serous ovarian carcinoma (HGSOC) and its correlation with clinical features. Methods: A total of 59 newly-diagnosed HGSOC patients, aged (58±11) years old, who were treated at the Department of Obstetrics and Gynecology of Peking University Third Hospital from July 2019 to December 2020 were included, collecting ascites and peritoneal irrigation fluid respectively. Detect the proportion of T, B, NK cell and its subpopulations by flow cytometry, and analyze its correlation with the clinical characteristics of patients. Results: Among 59 patients, 48 patients (81.4%) had ascites, and 11 patients (18.6%) had no ascites. Compared with the peritoneal irrigation fluid, the CD3+T (70.2%±15.6% vs 78.1%±6.7%, P=0.014), CD8+T (38.3%±11.2% vs 47.7%±10.1%, P=0.014) and CD16-CD56bright NK [2.0% (0.8%, 3.6%) vs 4.2% (1.5%, 7.1%), P=0.026] cells were significantly decreased in the ascites, while the CD16+CD56dim NK cells was significantly increased [6.8% (2.8%, 15.7%) vs 2.6% (1.6%, 4.3%), P=0.008]. In patients with ascites volume ≥1 000 ml, CD16-CD56bright NK cells were significantly increased than those ascites volume<1 000 ml [3.1% (1.2%, 3.9%) vs 0.8% (0.4%, 2.3%), P=0.002]. Age was significantly positively correlated with the level of CD4+T cells (r=0.332, P=0.021) and the ratio of CD4+T/CD8+T (r=0.379, P=0.008) in ascites. In ascites from patients with poor response to treatment, the proportion of CD4+T cells was increased significantly than those with better response (64.7%±4.2% vs 48.3%±11.7%, P=0.002). Conclusion: The formation of ascites in newly diagnosed high-grade serous epithelial ovarian cancer patients would affect the lymphocyte composition in the abdominal cavity microenvironment, which is related to the patient's age, cancer progression and treatment response.

目的: 分析高级别浆液性卵巢癌(HGSOC)腹水淋巴细胞组分及其与临床特征的相关性。 方法: 选取2019年7月至2020年12月于北京大学第三医院妇产科就诊的初治HGSOC患者59例,年龄为(58±11)岁,分别收集腹水及腹腔冲洗液。通过流式细胞术,检测其中T、B、自然杀伤(NK)细胞及其亚群的比例,并分析其与患者临床特征的相关性。 结果: 59例病例中有腹水者48例(81.4%),无腹水者11例(18.6%),腹水中的CD3+T(70.2%±15.6%比78.1%±6.7%,P=0.014)、CD8+T(38.3%±11.2%比47.7%±10.1%,P=0.014)和CD16-CD56bright NK[2.0%(0.8%,3.6%)比4.2%(1.5%,7.1%),P=0.026]细胞占比低于无腹水患者的腹腔冲洗液,而CD16+CD56dim NK细胞增多[6.8 %(2.8%,15.7%)比 2.6%(1.6%,4.3%),P=0.008]。与腹水量<1 000 ml的患者相比,腹水量≥1 000 ml的患者腹水中CD16-CD56bright NK细胞增多[3.1%(1.2%,3.9%)比 0.8%(0.4%,2.3%),P=0.002]。年龄与腹水中CD4+T 细胞水平(r=0.332,P=0.021)和CD4+T/CD8+T比值(r=0.379,P=0.008)均呈正相关。治疗疗效不佳的患者,腹水中CD4+T细胞多于疗效较好的患者(64.7%±4.2%比48.3%±11.7%,P=0.002)。 结论: 初治HGSOC患者腹水的形成会影响腹腔微环境中淋巴细胞的组分,其与患者年龄、癌症进展和治疗疗效存在相关性。.

MeSH terms

  • Aged
  • Ascites*
  • Female
  • Flow Cytometry
  • Humans
  • Killer Cells, Natural
  • Middle Aged
  • Ovarian Neoplasms*
  • T-Lymphocytes
  • Tumor Microenvironment