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. 1987;60(1):65-74.
doi: 10.1016/0378-1119(87)90214-9.

Cloning and Expression of the Mouse pgk-1 Gene and the Nucleotide Sequence of Its Promoter

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Cloning and Expression of the Mouse pgk-1 Gene and the Nucleotide Sequence of Its Promoter

C N Adra et al. Gene. .

Abstract

We report the cloning of the mouse pgk-1 gene encoding the somatic cell isoform of the enzyme phosphoglycerate kinase. The gene is contained within a 16-kb region of the X chromosome and is interrupted by at least ten introns. The promoter region of the pgk-1 gene is rich in G and C nucleotides and contains five copies of the hexadeoxynucleotide, GGGCGG, the potential binding site for the Sp 1 transcription factor, a CCAAT sequence, but no TATA box. This promoter functions following DNA-mediated transfection into mammalian cells. The promoter of the mouse pgk-1 gene is homologous to the human pgk-1 promoter. A number of conserved motifs in the promoter may indicate a significant role for these sequences in expression of the pgk-1 gene.

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