Cloning and expression of the mouse pgk-1 gene and the nucleotide sequence of its promoter

Gene. 1987;60(1):65-74. doi: 10.1016/0378-1119(87)90214-9.


We report the cloning of the mouse pgk-1 gene encoding the somatic cell isoform of the enzyme phosphoglycerate kinase. The gene is contained within a 16-kb region of the X chromosome and is interrupted by at least ten introns. The promoter region of the pgk-1 gene is rich in G and C nucleotides and contains five copies of the hexadeoxynucleotide, GGGCGG, the potential binding site for the Sp 1 transcription factor, a CCAAT sequence, but no TATA box. This promoter functions following DNA-mediated transfection into mammalian cells. The promoter of the mouse pgk-1 gene is homologous to the human pgk-1 promoter. A number of conserved motifs in the promoter may indicate a significant role for these sequences in expression of the pgk-1 gene.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular*
  • Electrophoresis, Agar Gel
  • Gene Expression Regulation*
  • HeLa Cells
  • Mice
  • Molecular Sequence Data
  • Phosphoglycerate Kinase / genetics*
  • Promoter Regions, Genetic*
  • Transfection*


  • Phosphoglycerate Kinase

Associated data

  • GENBANK/M18735