Isolation of Protein Complexes Associated with Long Non-coding RNAs

Min Li; Ying Xue; Ruiping He; Qihong Huang

doi:10.1007/978-1-0716-1697-0_4

Isolation of Protein Complexes Associated with Long Non-coding RNAs

Methods Mol Biol. 2021:2372:27-33. doi: 10.1007/978-1-0716-1697-0_4.

Authors

Min Li¹, Ying Xue¹, Ruiping He¹, Qihong Huang²

Affiliations

¹ Fudan University Zhongshan Hospital, Shanghai, P. R. China.
² Fudan University Zhongshan Hospital, Shanghai, P. R. China. qihong_huang@fudan.edu.cn.

PMID: 34417740
DOI: 10.1007/978-1-0716-1697-0_4

Abstract

Long non-coding RNAs (lncRNAs) are a new class of regulatory genes that play critical roles in various processes ranging from normal development to human diseases. Recent studies have shown that protein complexes are required for the functions of lncRNAs. The identification of these proteins which are associated with lncRNAs is critical for the understanding of molecular mechanisms of lncRNAs in gene regulation and their functions. In this chapter, we describe a method to isolate proteins associated with lncRNAs. This procedure involves fusion protein Maltose-Binding Protein (MBP) fused to MS2-binding protein to pull down the proteins associated with lncRNA and the identification of these proteins by mass spectrometry.

Keywords: Long non-coding RNA; MS2; Protein complex.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Gene Expression Regulation
Humans
Mass Spectrometry
RNA, Long Noncoding / genetics*

Substances

RNA, Long Noncoding