Differential expression of urine-circulating micro-RNAs in Chlamydia trachomatis-induced recurrent spontaneous aborters

Ankita Ray; Tanu Bhati; Renu Arora; Dibyabhaba Pradhan; Suhel Parvez; Sangita Rastogi

doi:10.1016/j.micpath.2021.105156

Differential expression of urine-circulating micro-RNAs in Chlamydia trachomatis-induced recurrent spontaneous aborters

Microb Pathog. 2021 Nov:160:105156. doi: 10.1016/j.micpath.2021.105156. Epub 2021 Aug 19.

Authors

Ankita Ray¹, Tanu Bhati¹, Renu Arora², Dibyabhaba Pradhan³, Suhel Parvez⁴, Sangita Rastogi⁵

Affiliations

¹ Molecular Microbiology Laboratory, ICMR-National Institute of Pathology, Sriramachari Bhawan, Safdarjung Hospital Campus, Post Box No. 4909, New Delhi, 110029, India.
² Department of Obstetrics and Gynaecology, Vardhman Mahavir Medical College (VMMC) and Safdarjung Hospital, New Delhi, 110029, India.
³ ICMR Computational Genomics Centre, All India Institute of Medical Sciences, New Delhi, 110029, India.
⁴ Department of Medical Elementology and Toxicology, Jamia Hamdard, New Delhi, 110062, India.
⁵ Molecular Microbiology Laboratory, ICMR-National Institute of Pathology, Sriramachari Bhawan, Safdarjung Hospital Campus, Post Box No. 4909, New Delhi, 110029, India. Electronic address: rastogi_sangita@rediffmail.com.

PMID: 34418493
DOI: 10.1016/j.micpath.2021.105156

Abstract

Studies behind mechanisms of Chlamydia trachomatis-induced recurrent spontaneous abortion is still in its infancy. Possible strategy for preventing recurrent spontaneous abortion at molecular level is needed. Despite its multifactorial aetiology, Chlamydia trachomatis is important cause of RSA. However, mechanism leading to RSA in C. trachomatis-positive patients is not understood and novel strategies are needed. It is hypothesized that microRNAs play important role in RSA regulation during infection. Study aimed to elucidate expression/role of urine-circulating miRs-320b, 221-3p, 146b-5p,-16,-24,-559 in recurrent spontaneous aborters with C. trachomatis infection and to find their target genes by bioinformatic analysis. First-void urine was collected from 30 non-pregnant women with RSA (Group I) and 30 non-pregnant women with ≥2 successful deliveries (Group II; Controls) attending Department of Obstetrics and Gynaecology, Vardhman Mahavir Medical College, Safdarjung hospital, New Delhi (India). PCR was performed to detect C. trachomatis. Expression of miRNAs was studied by quantitative real-time PCR while target genes/functional annotations were predicted by GO/KEGG databases. Data was statistically evaluated. 05 RSA patients were C. trachomatis-positive. Group I was subdivided into Group Ia (C. trachomatis-positive RSA; n = 5) and Group Ib (C. trachomatis-negative RSA; internal controls). miR-320b, -221-3p, -146b-5p, -16, -24 were significantly upregulated (miR-16 showed maximum 4.3 fold-change) while miR-559 was downregulated (0.5 fold-change) in Group Ia versus controls ('p'<0.001). Bioinformatic analysis revealed that target genes of miRNAs in RSA are involved in apoptosis and AMPK signalling pathways. Results showed differential expression of miRNAs implyingmiR-16 and miR-559 as potential biomarkers of RSA in infected women. Furthermore, network of genes of differentially expressed miRNAs regulates RSA by targeting gene function in apoptosis, cell adhesion and angiogenesis.

Keywords: Bioinformatic analysis; Chlamydia trachomatis; Quantitative real time PCR; Recurrent spontaneous abortion; Urine; microRNA.

MeSH terms

Abortion, Habitual* / genetics
Abortion, Habitual* / microbiology
Chlamydia trachomatis / pathogenicity*
Female
Humans
India
MicroRNAs* / genetics
MicroRNAs* / urine
Pregnancy

Substances

MicroRNAs