Identification method for twelve oligomannose-type sugar chains thought to be processing intermediates of glycoproteins

Anal Biochem. 1987 Dec;167(2):321-6. doi: 10.1016/0003-2697(87)90171-0.


A mixture of the pyridylamino (PA) derivatives of 12 oligomannose-type sugar chains was fractionated into five fractions (mannose5N-acetylglucosamine2-PA approximately mannose9N-acetylglucosamine2-PA) by size-fractionation HPLC with a MicroPak AX-5 column. Each fraction thus obtained was then analyzed by reversed-phase HPLC with a Cosmosil 5C18-P column. In this way, the 12 PA-oligomannose-type sugar chains were completely separated from each other. The method was used to identify the structures of oligomannose-type sugar chains of human C3, the third component of human complement.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbohydrate Sequence
  • Chromatography, High Pressure Liquid
  • Complement C3 / isolation & purification
  • Glycoproteins / isolation & purification*
  • Glycosides / isolation & purification*
  • Humans
  • Mannosides / isolation & purification*
  • Molecular Sequence Data
  • Oligosaccharides / isolation & purification*


  • Complement C3
  • Glycoproteins
  • Glycosides
  • Mannosides
  • Oligosaccharides