Protocol for the generation of blastocyst-like structures from mouse extended pluripotent stem cells

STAR Protoc. 2021 Aug 13;2(3):100745. doi: 10.1016/j.xpro.2021.100745. eCollection 2021 Sep 17.


Extended/expanded pluripotent stem (EPS) cells can efficiently contribute to both embryonic and extraembryonic lineages in vitro and in vivo. Starting from these cells, we established a 3D differentiation system that enabled the generation of blastocyst-like structures (EPS-blastoids) through lineage segregation and self-organization. We also provide proof of concept that EPS-blastoids can be generated from adult cells via cellular reprogramming. EPS-blastoids provide a unique platform for studying early embryogenesis. For complete details on the use and execution of this protocol, please refer to Li et al. (2019).

Keywords: Cell Biology; Cell Differentiation; Cell culture; Developmental biology; Organoids; Stem Cells.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blastocyst / cytology
  • Blastocyst / metabolism
  • Blastocyst / physiology
  • Cell Culture Techniques / methods*
  • Cell Differentiation
  • Cell Lineage
  • Embryo Culture Techniques / methods*
  • Embryonic Development
  • Mice
  • Pluripotent Stem Cells / cytology
  • Pluripotent Stem Cells / metabolism