Acute regulation of insulin-like growth factor-I gene expression by growth hormone during adipose cell differentiation

EMBO J. 1987 Dec 20;6(13):4011-6.

Abstract

Insulin-like growth factor I (IGF-I) is a mitogenic polypeptide that is thought to play, under the control of growth hormone, a role in fetal development as well as post-natally. The direct effect of growth hormone on the regulation of the expression of IGF-I gene was examined in adipose Ob1771 cells. Growth hormone regulates the abundance of multiple species of IGF-I mRNAs of 15, 7.5, 1.5 and 0.8 kb in a differentiation-dependent manner. The regulation of IGF-I gene expression is strikingly rapid (less than 2 h), reversible and takes place primarily at transcriptional level. Thus growth hormone can increase the cellular content of IGF-I mRNA encoding for a protein which could be involved in a paracrine/autocrine action during adipose tissue development.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipose Tissue / cytology
  • Adipose Tissue / drug effects
  • Adipose Tissue / metabolism*
  • Animals
  • Cell Differentiation
  • Cells, Cultured
  • Gene Expression Regulation / drug effects*
  • Genes / drug effects*
  • Insulin-Like Growth Factor I / genetics*
  • Male
  • RNA, Messenger / drug effects
  • RNA, Messenger / genetics
  • Rats
  • Rats, Inbred Strains
  • Somatomedins / genetics*
  • Transcription, Genetic / drug effects

Substances

  • RNA, Messenger
  • Somatomedins
  • Insulin-Like Growth Factor I