Metabolomic and lipidomic signatures associated with activation of human cDC1 (BDCA3+ /CD141+ ) dendritic cells

Immunology. 2022 Jan;165(1):99-109. doi: 10.1111/imm.13409. Epub 2021 Sep 7.


Dendritic cells (DCs) bridge the connection between innate and adaptive immunity. DCs present antigens to T cells and stimulate potent cytotoxic T-cell responses. Metabolic reprogramming is critical for DC development and activation; however, metabolic adaptations and regulation in DC subsets remains largely uncharacterized. Here, we mapped metabolomic and lipidomic signatures associated with the activation phenotype of human conventional DC type 1, a DC subset specialized in cross-presentation and therefore of major importance for the stimulation of CD8+ T cells. Our metabolomics and lipidomic analyses showed that Toll-like receptor (TLR) stimulation altered glycerolipids and amino acids in cDC1. Poly I:C or pRNA stimulation reduced triglycerides and cholesterol esters, as well as various amino acids. Moreover, TLR stimulation reduced expression of glycolysis-regulating genes and did not induce glycolysis. Conversely, cDC1 exhibited increased mitochondrial content and oxidative phosphorylation (OXPHOS) upon TLR3 or TLR7/8 stimulation. Our findings highlight the metabolic adaptations required for cDC1 maturation.

Keywords: BDCA3; dendritic cell activation; lipid mediators; metabolomics.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acids / metabolism
  • Biomarkers
  • Cytokines / metabolism
  • Dendritic Cells / immunology*
  • Dendritic Cells / metabolism*
  • Humans
  • Immunophenotyping
  • Lipid Metabolism*
  • Lipidomics* / methods
  • Lipopolysaccharide Receptors / metabolism
  • Metabolic Networks and Pathways
  • Metabolome
  • Metabolomics
  • Oxidative Phosphorylation
  • Thrombomodulin / metabolism
  • Toll-Like Receptors / metabolism


  • Amino Acids
  • Biomarkers
  • Cytokines
  • Lipopolysaccharide Receptors
  • THBD protein, human
  • Thrombomodulin
  • Toll-Like Receptors