Design and construction of a versatile system for the expression of foreign genes in plants

Gene. 1987;61(1):1-11. doi: 10.1016/0378-1119(87)90359-3.


We have built a series of vectors to allow the constitutive or light-regulated expression of foreign genes in plants. These vectors carry expression cassettes consisting of either the cauliflower mosaic virus 35S promoter or the pea rbcS-E9 promoter, a multiple cloning site derived from M13um20, and the rbcS-E9 polyadenylation site. These cassettes have been incorporated into pBR322-based or RK2-based replicons to facilitate direct DNA uptake or Agrobacterium tumefaciens-mediated gene transfer. Their application for the expression of a bacterial gene is described.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Base Sequence
  • Cloning, Molecular
  • DNA / genetics
  • Genetic Vectors*
  • Molecular Sequence Data
  • Mosaic Viruses / genetics
  • Plants / genetics*
  • Promoter Regions, Genetic
  • Replicon
  • Rhizobium / genetics
  • Transfection


  • DNA

Associated data

  • GENBANK/M19099