The diagnostic applicability of A-type Lamin in non-muscle invasive bladder cancer

Nouha Setti Boubaker; Aymone Gurtner; Nesrine Trabelsi; Isabella Manni; Haroun Ayed; Ahmed Saadi; Selim Zaghbib; Zeineb Naimi; Ghada Sahraoui; Skander Zouari; Khedija Meddeb; Karima Mrad; Mohamed Chebil; Giulia Piaggio; Slah Ouerhani

doi:10.1016/j.anndiagpath.2021.151808

The diagnostic applicability of A-type Lamin in non-muscle invasive bladder cancer

Ann Diagn Pathol. 2021 Oct:54:151808. doi: 10.1016/j.anndiagpath.2021.151808. Epub 2021 Aug 17.

Authors

Nouha Setti Boubaker¹, Aymone Gurtner², Nesrine Trabelsi³, Isabella Manni⁴, Haroun Ayed⁵, Ahmed Saadi⁶, Selim Zaghbib⁷, Zeineb Naimi⁸, Ghada Sahraoui⁹, Skander Zouari¹⁰, Khedija Meddeb¹¹, Karima Mrad¹², Mohamed Chebil¹³, Giulia Piaggio¹⁴, Slah Ouerhani¹⁵

Affiliations

¹ Laboratory of Proteins Engineering and Bioactive Molecules (LIP-MB), INSAT, University of Tunis Carthage, Tunis, Tunisia; UOSD SAFU, Department of Research, Diagnosis and Innovative Technologies, IRCCS-Regina Elena National Cancer Institute, Rome, Italy. Electronic address: nouha.setti@hotmail.fr.
² UOSD SAFU, Department of Research, Diagnosis and Innovative Technologies, IRCCS-Regina Elena National Cancer Institute, Rome, Italy; Institute of Translational Pharmacology, National Research Council, Rome, Italy. Electronic address: aymone.gurtner@ifo.gov.it.
³ Laboratory of Proteins Engineering and Bioactive Molecules (LIP-MB), INSAT, University of Tunis Carthage, Tunis, Tunisia. Electronic address: nesrine.trabelsi.n@ieee.org.
⁴ UOSD SAFU, Department of Research, Diagnosis and Innovative Technologies, IRCCS-Regina Elena National Cancer Institute, Rome, Italy. Electronic address: isabella.manni@ifo.gov.it.
⁵ Urology Department, Charles Nicolle Hospital, Faculty of Medicine of Tunis, University of Tunis-El Manar, Tunis, Tunisia. Electronic address: ayedharoun@yahoo.fr.
⁶ Urology Department, Charles Nicolle Hospital, Faculty of Medicine of Tunis, University of Tunis-El Manar, Tunis, Tunisia. Electronic address: dr.saadi.ahmed@gmail.com.
⁷ Urology Department, Charles Nicolle Hospital, Faculty of Medicine of Tunis, University of Tunis-El Manar, Tunis, Tunisia. Electronic address: selim.zaghbib@gmail.com.
⁸ Medical Oncology Department, Salah Azaiez Institute, Faculty of Medicine of Tunis, University of Tunis-El Manar, Tunis, Tunisia. Electronic address: zeinebnaimi89@gmail.com.
⁹ Pathology Department, Salah Azaiez Institute, Faculty of Medicine of Tunis, University of Tunis-El Manar, Tunis, Tunisia. Electronic address: ghada_sahraoui@yahoo.com.
¹⁰ Urology Department, Charles Nicolle Hospital, Faculty of Medicine of Tunis, University of Tunis-El Manar, Tunis, Tunisia. Electronic address: zouari.skander@gmail.com.
¹¹ Medical Oncology Department, Salah Azaiez Institute, Faculty of Medicine of Tunis, University of Tunis-El Manar, Tunis, Tunisia. Electronic address: khedijameddeb@gmail.com.
¹² Pathology Department, Salah Azaiez Institute, Faculty of Medicine of Tunis, University of Tunis-El Manar, Tunis, Tunisia. Electronic address: karima.mrad@rns.tn.
¹³ Urology Department, Charles Nicolle Hospital, Faculty of Medicine of Tunis, University of Tunis-El Manar, Tunis, Tunisia. Electronic address: mohamed.chebil.uro@gmail.com.
¹⁴ UOSD SAFU, Department of Research, Diagnosis and Innovative Technologies, IRCCS-Regina Elena National Cancer Institute, Rome, Italy. Electronic address: giulia.piaggio@ifo.gov.it.
¹⁵ Laboratory of Proteins Engineering and Bioactive Molecules (LIP-MB), INSAT, University of Tunis Carthage, Tunis, Tunisia. Electronic address: slah_mekni@yahoo.fr.

PMID: 34438192
DOI: 10.1016/j.anndiagpath.2021.151808

Abstract

Purpose: Lamin A is a major component of the nuclear lamina maintaining nuclear integrity, regulation of gene expression, cell proliferation, and apoptosis. Its deregulation in cancer has been recently reported to be associated with its prognosis. However, its clinical significance in non-muscle invasive bladder cancer (NMIBC) remains to be defined.

Material/methods: Immunohistochemical staining and RT-qPCR were performed to screen the expression patterns of Lamin A/C protein and Lamin A mRNA respectively in 58 high and low grade NMIBC specimens.

Results: Lamin A/C protein was expressed only in the nucleus and less exhibited in NMIBC tissues compared to non-tumoral ones. On the other side, Lamin A mRNA was up-regulated in NMIBC compared to controls. Nevertheless, both expression patterns (protein and mRNA) were not correlated to clinical prognosis factors and were not able to predict the overall survival of patients with high-grade NMIBC.

Conclusions: The deregulation of A-type Lamin is not associated with the prognosis of NMIBC, but it could serve as a diagnostic biomarker distinguishing NMIBC patients from healthy subjects suggesting its involvement as an initiator event of tumorigenesis in our cohort.

Keywords: Diagnosis; LMNA; Lamin A/C; NMIBC; Prognosis.

MeSH terms

Biomarkers, Tumor / metabolism
Female
Gene Expression Regulation, Neoplastic / genetics
Humans
Lamins / genetics
Lamins / metabolism*
Male
Middle Aged
Neoplasm Invasiveness / genetics*
Neoplasm Invasiveness / pathology*
Neoplasm Recurrence, Local / diagnosis
Neoplasm Recurrence, Local / genetics*
Neoplasm Recurrence, Local / pathology
Prognosis
RNA, Messenger / genetics
Urinary Bladder Neoplasms* / diagnosis
Urinary Bladder Neoplasms* / genetics
Urinary Bladder Neoplasms* / metabolism

Substances

Biomarkers, Tumor
Lamins
RNA, Messenger