Evaluation of the Free Radical Scavenging Activities of Ellagic Acid and Ellagic Acid Peracetate by EPR Spectrometry

Molecules. 2021 Aug 8;26(16):4800. doi: 10.3390/molecules26164800.


The purpose of this study was to examine the free radical scavenging and antioxidant activities of ellagic acid (EA) and ellagic acid peracetate (EAPA) by measuring their reactions with the radicals, 2,2-diphenyl-1-picrylhydrazyl and galvinoxyl using EPR spectroscopy. We have also evaluated the influence of EA and EAPA on the ROS production in L-6 myoblasts and rat liver microsomal lipid peroxidation catalyzed by NADPH. The results obtained clearly indicated that EA has tremendous ability to scavenge free radicals, even at concentration of 1 µM. Interestingly even in the absence of esterase, EAPA, the acetylated product of EA, was also found to be a good scavenger but at a relatively slower rate. Kinetic studies revealed that both EA and EAPA have ability to scavenge free radicals at the concentrations of 1 µM over extended periods of time. In cellular systems, EA and EAPA were found to have similar potentials for the inhibition of ROS production in L-6 myoblasts and NADPH-dependent catalyzed microsomal lipid peroxidation.

Keywords: L-6 myoblasts; cytotoxicity; electron paramagnetic resonance; ellagic acid; ellagic acid peracetate; free radical.

MeSH terms

  • Animals
  • Electron Spin Resonance Spectroscopy*
  • Ellagic Acid / analogs & derivatives*
  • Ellagic Acid / pharmacology*
  • Free Radical Scavengers / pharmacology*
  • Kinetics
  • Microsomes, Liver / drug effects
  • Microsomes, Liver / metabolism
  • Peracetic Acid / analogs & derivatives*
  • Peracetic Acid / pharmacology
  • Rats


  • Free Radical Scavengers
  • ellagic acid peracetate
  • Ellagic Acid
  • Peracetic Acid