Keratin-Alginate Sponges Support Healing of Partial-Thickness Burns

Int J Mol Sci. 2021 Aug 10;22(16):8594. doi: 10.3390/ijms22168594.


Deep partial-thickness burns damage most of the dermis and can cause severe pain, scarring, and mortality if left untreated. This study serves to evaluate the effectiveness of crosslinked keratin-alginate composite sponges as dermal substitutes for deep partial-thickness burns. Crosslinked keratin-alginate sponges were tested for the ability to support human dermal fibroblasts in vitro and to support the closure and healing of partial-thickness burn wounds in Sus scrofa pigs. Keratin-alginate composite sponges supported the enhanced proliferation of human dermal fibroblasts compared to alginate-only sponges and exhibited decreased contraction in vitro when compared to keratin only sponges. As dermal substitutes in vivo, the sponges supported the expression of keratin 14, alpha-smooth muscle actin, and collagen IV within wound sites, comparable to collagen sponges. Keratin-alginate composite sponges supported the regeneration of basement membranes in the wounds more than in collagen-treated wounds and non-grafted controls, suggesting the subsequent development of pathological scar tissues may be minimized. Results from this study indicate that crosslinked keratin-alginate sponges are suitable alternative dermal substitutes for clinical applications in wound healing and skin regeneration.

Keywords: fibroblast; human hair keratin; porcine burn wounds; sponge; wound healing.

MeSH terms

  • Alginates / chemistry
  • Alginates / pharmacology
  • Alginates / therapeutic use*
  • Animals
  • Bandages, Hydrocolloid
  • Burns / pathology
  • Burns / physiopathology
  • Burns / therapy*
  • Cells, Cultured
  • Dermis / drug effects
  • Dermis / pathology
  • Dermis / physiopathology
  • Humans
  • Hydrogels / chemistry
  • Hydrogels / therapeutic use
  • Keratins / chemistry
  • Keratins / pharmacology
  • Keratins / therapeutic use*
  • Male
  • Materials Testing
  • Severity of Illness Index
  • Skin / drug effects
  • Skin / pathology
  • Skin / physiopathology
  • Swine
  • Wound Healing* / drug effects
  • Wound Healing* / physiology


  • Alginates
  • Hydrogels
  • Keratins