Changes in Phospholipid/Ceramide Profiles and Eicosanoid Levels in the Plasma of Rats Irradiated with UV Rays and Treated Topically with Cannabidiol

Int J Mol Sci. 2021 Aug 13;22(16):8700. doi: 10.3390/ijms22168700.


Chronic UV radiation causes oxidative stress and inflammation of skin and blood cells. Therefore, in this study, we assessed the effects of cannabidiol (CBD), a natural phytocannabinoid with antioxidant and anti-inflammatory properties, on the phospholipid (PL) and ceramide (CER) profiles in the plasma of nude rats irradiated with UVA/UVB and treated topically with CBD. The results obtained showed that UVA/UVB radiation increased the levels of phosphatidylcholines, lysophospholipids, and eicosanoids (PGE2, TxB2), while downregulation of sphingomyelins led to an increase in CER[NS] and CER[NDS]. Topical application of CBD to the skin of control rats significantly upregulated plasma ether-linked phosphatidylethanolamines (PEo) and ceramides. However, CBD administered to rats irradiated with UVA/UVB promoted further upregulation of CER and PEo and led to significant downregulation of lysophospholipids. This was accompanied by the anti-inflammatory effect of CBD, manifested by a reduction in the levels of proinflammatory PGE2 and TxB2 and a dramatic increase in the level of anti-inflammatory LPXA4. It can therefore be suggested that topical application of CBD to the skin of rats exposed to UVA/UVB radiation prevents changes in plasma phospholipid profile resulting in a reduction of inflammation by reducing the level of LPE and LPC species and increasing antioxidant capacity due to upregulation of PEo species.

Keywords: UV irradiation; cannabidiol; ceramides; eicosanoids; lipidomics; phospholipids; plasma; rats.

MeSH terms

  • Administration, Topical
  • Animals
  • Cannabidiol / administration & dosage*
  • Cannabidiol / pharmacology
  • Ceramides / blood*
  • Ceramides / radiation effects
  • Chromatography, Reverse-Phase
  • Eicosanoids / blood*
  • Eicosanoids / radiation effects
  • Male
  • Phospholipids / blood*
  • Phospholipids / radiation effects
  • Rats
  • Rats, Nude
  • Tandem Mass Spectrometry
  • Ultraviolet Rays / adverse effects*


  • Ceramides
  • Eicosanoids
  • Phospholipids
  • Cannabidiol