MicroRNA-590-3p relieves hypoxia/reoxygenation induced cardiomyocytes apoptosis and autophagy by targeting HIF-1α

Nengjing Gong; Xiaohuai Yang; Xiaoyu Li; Yuyu Jiang; Cuifang Gu; Shasha Ma; Qin Gao; Xiangyang Cheng

doi:10.3892/etm.2021.10511

MicroRNA-590-3p relieves hypoxia/reoxygenation induced cardiomyocytes apoptosis and autophagy by targeting HIF-1α

Exp Ther Med. 2021 Oct;22(4):1077. doi: 10.3892/etm.2021.10511. Epub 2021 Jul 28.

Authors

Nengjing Gong¹, Xiaohuai Yang², Xiaoyu Li¹, Yuyu Jiang¹, Cuifang Gu¹, Shasha Ma¹, Qin Gao³, Xiangyang Cheng¹

Affiliations

¹ Department of Anesthesiology, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233000, P.R. China.
² Department of Urology, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233000, P.R. China.
³ Department of Physiology, Key Laboratory of Basic and Clinical Cardiovascular Diseases, Bengbu Medical College, Bengbu, Anhui 233000, P.R. China.

Abstract

Autophagy and apoptosis are key factors in myocardial ischemia/reperfusion (I/R) injury. MicroRNAs (miRNAs or miRs) participate in occurrence and development of myocardial I/R injury by regulating autophagy and apoptosis. The purpose of the present study was to investigate the role of miR-590-3p in the regulation of autophagy and apoptosis in hypoxia/reoxygenation (H/R)-treated cardiomyocytes. Following 6 h hypoxia and 6 h reoxygenation in primary rat cardiomyocytes, miR-590-3p was downregulated. Transfection of miR-590-3p mimic inhibited the increased autophagy and apoptosis following H/R treatment. Subsequent experiments demonstrated that miR-590-3p regulated induction of autophagy and apoptosis by targeting hypoxia inducible factor (HIF)-1α. Forced expression of HIF-1α rescued the protective effect of miR-590-3p on H/R-induced cardiomyocytes. In summary, the present study showed that miR-590-3p exhibited a protective effect on H/R-induced cardiomyocyte injury and may be a novel target for the treatment of myocardial ischemia disease.

Keywords: apoptosis; autophagy; cardiomyocyte; hypoxia inducible factor-1α; hypoxia/reoxygenation; microRNA-590-3p.

Grants and funding

Funding: The present study was supported by grants from the National Natural Science Foundation of China (grant no. 81770297) and the Nature Science Major and Key Program of College and University of Anhui Province (grant no. KJ2018A0212).