QuinoMit Q10-Fluid attenuates hydrogen peroxide-induced irregular beating in mouse pluripotent stem cell-derived cardiomyocytes

Biomed Pharmacother. 2021 Oct;142:112089. doi: 10.1016/j.biopha.2021.112089. Epub 2021 Aug 25.

Abstract

Background: Coenzyme Q10 (CoQ10) is a crucial component of the mitochondrial structure which is involved in producing more than 90% of cellular ATP. This study aimed to investigate the protective effects and underlying mechanisms of QuinoMit Q10-Fluid against hydrogen peroxide (H2O2)-induced arrhythmias on cardiomyocytes (CMs).

Methods: Undifferentiated stem cell-derived CMs were cultured in the presence of different concentrations of QuinoMit Q10-Fluid. To investigate if CoQ10 has anti-apoptotic activity, CMs were exposed to H2O2 for up to 100 h with or without CoQ10. The expression levels of cardiac reference genes were determined by RT-PCR. The structural and functional properties of CMs were examined by immunofluorescence and the xCELLigence system. Caspase 3/7 assay was also performed for cell apoptosis study.

Results: The study showed that QuinoMit Q10-Fluid inhibits the proliferation of pluripotent stem cells at high concentrations and had less effect on cardiomyogenesis. However, the beating rate of clusters containing CMs generated under QuinoMit Q10-Fluid (1:100) was significantly increased. This increase was accompanied by the up-regulated expression level of some important cardiac markers during differentiation. Treatment of CMs with H2O2 notably induced irregular beating and decreased the amplitude of the beating signal of CMs, concomitantly with increased caspase-3/7 activity. However, CMs pretreated with QuinoMit exhibited a protective effect against H2O2-induced arrhythmia.

Conclusion: Our results reveal that QuinoMit Q10-Fluid attenuates H2O2-induced irregular beating in mouse pluripotent stem cell-derived CMs, at least partly by reducing the generation of ROS, suggesting a protective effect against CM dysfunctions.

Keywords: Apoptosis; Arrhythmia; Cardiomyocytes; Cell Index; Induced pluripotent stem cells; QuinoMit Q10-Fluid; xCELLigence.

MeSH terms

  • Animals
  • Apoptosis / drug effects
  • Arrhythmias, Cardiac / prevention & control*
  • Cardiotonic Agents / pharmacology*
  • Cell Differentiation / physiology
  • Cell Proliferation / drug effects
  • Cells, Cultured
  • Hydrogen Peroxide
  • Induced Pluripotent Stem Cells / cytology
  • Mice
  • Mice, Transgenic
  • Myocytes, Cardiac / drug effects*
  • Myocytes, Cardiac / pathology
  • Ubiquinone / analogs & derivatives*
  • Ubiquinone / pharmacology

Substances

  • Cardiotonic Agents
  • Ubiquinone
  • Hydrogen Peroxide
  • coenzyme Q10