Three-step procedure for isolation of epithelial cells from the lungs of sheep with jaagsiekte

Res Vet Sci. 1987 Nov;43(3):407-9.

Abstract

An efficient and reproducible technique is described for the isolation of transformed sheep pulmonary adenomatosis cells. It includes three basic steps: prolonged trypsinisation to kill fibroblasts, magnetic removal of macrophages and adherence to remove the rapidly adherent cells. The resultant preparations of lung cells were enriched to 96.6 per cent type 2 pneumocytes.

MeSH terms

  • Animals
  • Cell Separation / methods*
  • Epithelium / pathology
  • Lung / pathology*
  • Pulmonary Adenomatosis, Ovine / pathology*
  • Sheep