Isolation of Outer Membrane Vesicles from Phytopathogenic Xanthomonas campestris pv. campestris

Gideon Mordukhovich; Ofir Bahar

doi:10.21769/BioProtoc.2160

Isolation of Outer Membrane Vesicles from Phytopathogenic Xanthomonas campestris pv. campestris

Bio Protoc. 2017 Mar 5;7(5):e2160. doi: 10.21769/BioProtoc.2160.

Authors

Gideon Mordukhovich^{1

2}, Ofir Bahar¹

Affiliations

¹ Department of Plant Pathology and Weed Research, Agricultural Research Organization, Volcani Center, Rishon LeZion, Israel.
² Robert H. Smith Faculty of Agriculture, Food, and Environment, Hebrew University of Jerusalem, Rehovot, Israel.

Abstract

Gram-negative bacteria naturally release outer membrane vesicles (OMVs) to the surrounding environment. OMVs contribute to multiple processes, such as cell-cell communication, delivery of enzymes and toxins, resistance to environmental stresses and pathogenesis. Little is known about OMVs produced by plant-pathogenic bacteria, and their interactions with host plants. The protocol described below discusses the isolation process of OMVs from Xanthomonas campestris pv. campestris strain 33913, a bacterial pathogen of Crucifiers. Nevertheless, this protocol can be used and/or adapted for isolation of OMVs from other phytopathogenic bacteria to promote the study of OMVs in the context of plant-microbe interactions.

Keywords: Extracellular vesicles; Isolation; OMVs; Outer membrane vesicles; Plant-microbe interactions; Purification; Xanthomonas campestris pv. campestris.