Visualizing murine breast and melanoma tumor microenvironment using intravital multiphoton microscopy

Rohit Jain; Shweta Tikoo; Kathy On; Brendon Martinez; Suat Dervish; Lois L Cavanagh; Wolfgang Weninger

doi:10.1016/j.xpro.2021.100722

Visualizing murine breast and melanoma tumor microenvironment using intravital multiphoton microscopy

STAR Protoc. 2021 Aug 19;2(3):100722. doi: 10.1016/j.xpro.2021.100722. eCollection 2021 Sep 17.

Authors

Rohit Jain¹, Shweta Tikoo¹, Kathy On¹, Brendon Martinez¹, Suat Dervish¹, Lois L Cavanagh¹, Wolfgang Weninger^{1

2}

Affiliations

¹ Centenary Institute, Faculty of Medicine and Health, The University of Sydney, Camperdown, NSW 2050, Australia.
² Department of Dermatology, Medical University of Vienna, Vienna 1090, Austria.

Abstract

Intravital multiphoton imaging of the tumor milieu allows for the dissection of intricate and dynamic biological processes in situ. Herein, we present a step-by-step protocol for setting up an experimental cancer imaging model that has been optimized for solid tumors such as breast cancer and melanoma implanted in the flanks of mice. This protocol can be utilized for dissecting tumor-immune cell dynamics in vivo or other tumor-specific biological questions. For complete details on the use of this protocol for intravital imaging of breast cancer, please refer to Tikoo et al. (2021a), and for intravital imaging of melanoma, please refer to Tikoo et al. (2021b).

Keywords: Cancer; Immunology; Microscopy.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Breast Neoplasms / diagnostic imaging
Female
Intravital Microscopy / methods*
Melanoma / diagnostic imaging
Mice
Microscopy, Fluorescence, Multiphoton / methods*
Tumor Microenvironment / physiology*