Isolation and characterization of a naturally attenuated novel duck reovirus strain as a live vaccine candidate

Hui Yan; Guige Xu; Yanli Zhu; Zhijing Xie; Ruihua Zhang; Shijin Jiang

doi:10.1016/j.vetmic.2021.109214

Isolation and characterization of a naturally attenuated novel duck reovirus strain as a live vaccine candidate

Vet Microbiol. 2021 Oct:261:109214. doi: 10.1016/j.vetmic.2021.109214. Epub 2021 Aug 25.

Authors

Hui Yan¹, Guige Xu¹, Yanli Zhu¹, Zhijing Xie¹, Ruihua Zhang², Shijin Jiang³

Affiliations

¹ Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Taian, 271018, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Taian, 271018, China; Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Taian, 271018, China.
² Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Taian, 271018, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Taian, 271018, China; Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Taian, 271018, China. Electronic address: rhzhang@sdau.edu.cn.
³ Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Taian, 271018, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Taian, 271018, China; Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Taian, 271018, China. Electronic address: sjjiang@sdau.edu.cn.

PMID: 34461358
DOI: 10.1016/j.vetmic.2021.109214

Abstract

Novel duck reovirus (NDRV) causes high morbidity in ducklings, and recovered ducklings are often remarkably stunted in growth. In this study, four NDRV strains were isolated from the NDRV outbreaks that occurred in different regions of Shandong province, China. The biological characteristics and pathogenicity of the four NDRV strains were elucidated, and the N20 was identified as a naturally attenuated strain. Three-day-old ducklings were immunized with live N20 strain (100 ELD₅₀/duck), and challenged with 10^4.52 ELD₅₀ of virulent N19 strain at 7 days post immunization. The vaccinated ducklings showed no evidence of clinical signs, gross and histopathological lesions, or loss of body weight, and 100 % protection against the virulent NDRV N19 infection. The NDRV-specific antibodies were generated in the immunized ducklings and could neutralize different NDRV strains. These results indicated that the N20 strain was a promising live attenuated vaccine candidate against highly pathogenic NDRV infection.

Keywords: Isolation; Live attenuated vaccine; Novel duck reovirus; Protection.

MeSH terms

Animals
Antibodies, Neutralizing / metabolism
Antibodies, Viral / blood
China
Ducks
Orthoreovirus, Avian / genetics
Orthoreovirus, Avian / immunology*
Orthoreovirus, Avian / isolation & purification
Poultry Diseases / prevention & control*
Poultry Diseases / virology
Reoviridae Infections / prevention & control
Reoviridae Infections / veterinary*
Vaccines, Attenuated / immunology
Viral Vaccines / immunology*

Substances

Antibodies, Neutralizing
Antibodies, Viral
Vaccines, Attenuated
Viral Vaccines