Multifunctional microcapsules: A theranostic agent for US/MR/PAT multi-modality imaging and synergistic chemo-photothermal osteosarcoma therapy

Hufei Wang; Sijia Xu; Daoyang Fan; Xiaowen Geng; Guang Zhi; Decheng Wu; Hong Shen; Fei Yang; Xiao Zhou; Xing Wang

doi:10.1016/j.bioactmat.2021.05.004

Multifunctional microcapsules: A theranostic agent for US/MR/PAT multi-modality imaging and synergistic chemo-photothermal osteosarcoma therapy

Bioact Mater. 2021 May 29:7:453-465. doi: 10.1016/j.bioactmat.2021.05.004. eCollection 2022 Jan.

Authors

Hufei Wang^{1

2}, Sijia Xu^{1

2}, Daoyang Fan³, Xiaowen Geng⁴, Guang Zhi⁴, Decheng Wu^{1

2}, Hong Shen^{1

2}, Fei Yang^{1

2}, Xiao Zhou⁴, Xing Wang^{1

2}

Affiliations

¹ Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, China.
² University of Chinese Academy of Sciences, Beijing, 100049, China.
³ Department of Orthopaedic, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450000, China.
⁴ Department of Cardiology, Chinese PLA General Hospital, Beijing, 100853, China.

Abstract

Development of versatile theranostic agents that simultaneously integrate therapeutic and diagnostic features remains a clinical urgent. Herein, we aimed to prepare uniform PEGylated (lactic-co-glycolic acid) (PLGA) microcapsules (PB@(Fe₃O₄@PEG-PLGA) MCs) with superparamagnetic Fe₃O₄ nanoparticles embedded in the shell and Prussian blue (PB) NPs inbuilt in the cavity via a premix membrane emulsification (PME) method. On account of the eligible geometry and multiple load capacity, these MCs could be used as efficient multi-modality contrast agents to simultaneously enhance the contrasts of US, MR and PAT imaging. In-built PB NPs furnished the MCs with excellent photothermal conversion property and embedded Fe₃O₄ NPs endowed the magnetic location for fabrication of targeted drug delivery system. Notably, after further in-situ encapsulation of antitumor drug of DOX, (PB+DOX)@(Fe₃O₄@PEG-PLGA) MCs possessed more unique advantages on achieving near infrared (NIR)-responsive drug delivery and magnetic-guided chemo-photothermal synergistic osteosarcoma therapy. In vitro and in vivo studies revealed these biocompatible (PB+DOX)@(Fe₃O₄@PEG-PLGA) MCs could effectively target to the tumor tissue with superior therapeutic effect against the invasion of osteosarcoma and alleviation of osteolytic lesions, which will be developed as a smart platform integrating multi-modality imaging capabilities and synergistic effect with high therapy efficacy.

Keywords: Drug delivery; Microcapsule; Multi-modality imaging; Osteosarcoma; Photothermal therapy.