Microsatellite Markers in Biobanking: A New Multiplexed Assay

Biopreserv Biobank. 2021 Oct;19(5):438-443. doi: 10.1089/bio.2021.0042. Epub 2021 Sep 1.


Microsatellites, or MSATs, offer a fast and cost-effective way for biobanks to establish a biospecimen genetic profile. Importantly, this genetic profile can be used to authenticate multiple submissions derived from the same individual as well as biospecimens derived from the same original sample submission over time. While the Certificate of Confidentiality provided by the National Institutes of Health offers some meaningful protection to prevent the disclosure of potentially identifiable information to entities within the United States, we consider, in this study, the potential to offer additional protection to participants who choose to donate to biobanks by minimizing the use of forensic Combined DNA Index System (CODIS) MSAT markers in biobanking. To this end, we report the design and validation of a new multiplexed MSAT assay that does not include CODIS markers for use in biobanking operations and quality control management.

Keywords: biobanking; biospecimen authentication; microsatellite.

MeSH terms

  • Biological Specimen Banks*
  • Confidentiality*
  • Disclosure
  • Humans
  • Microsatellite Repeats
  • National Institutes of Health (U.S.)
  • United States