LC3A-mediated autophagy regulates lung cancer cell plasticity

Autophagy. 2022 Apr;18(4):921-934. doi: 10.1080/15548627.2021.1964224. Epub 2021 Sep 1.

Abstract

ATG14: autophagy related 14; CDH2: cadherin 2; ChIP-qPCR: chromatin immunoprecipitation quantitative polymerase chain reaction; CQ: chloroquine; ECAR: extracellular acidification rate; EMT: epithelial-mesenchymal transition; EPCAM: epithelial cell adhesion molecule; MAP1LC3A/LC3A: microtubule associated protein 1 light chain 3 alpha; MAP1LC3B/LC3B: microtubule associated protein 1 light chain 3 beta; MAP1LC3C/LC3C: microtubule associated protein 1 light chain 3 gamma; NDUFV2: NADH:ubiquinone oxidoreductase core subunit V2; OCR: oxygen consumption rate; ROS: reactive oxygen species; RT-qPCR: reverse-transcriptase quantitative polymerase chain reaction; SC: scrambled control; shRNA: short hairpin RNA; SNAI2: snail family transcriptional repressor 2; SOX2: SRY-box transcription factor 2; SQSTM1/p62: sequestosome 1; TGFB/TGF-β: transforming growth factor beta; TOMM20: translocase of outer mitochondrial membrane 20; ZEB1: zinc finger E-box binding homeobox 1.

Keywords: Autophagy; LC3A; SOX2; cancer cell plasticity; lung cancer; mitochondria dynamics.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Autophagy* / physiology
  • Cell Plasticity
  • Humans
  • Lung Neoplasms*
  • Microtubule-Associated Proteins / metabolism
  • Reactive Oxygen Species / metabolism

Substances

  • Microtubule-Associated Proteins
  • Reactive Oxygen Species

Grant support

This work was supported by National Tsing Hua University and Ministry of Science and Technology [106-2320-B-007-005-MY3 and 109-2320-B-007-003-MY3], Executive Yuan, Taiwan (R.O.C.).