In this study, we describe the development of an analytical method to profile naphthenic acids (NAs) from produced water (PW). The NAs were isolated by hollow fiber liquid-phase microextraction (HF-LPME). A microwave-assisted methylation method was used to convert the free acids into its corresponding naphthenic methyl esters (NAMEs). The best reaction conditions were ascertained using central composite design. The optimized sample preparation method exhibited an improved analytical eco-scale value (80 vs. 61) compared to conventional liquid-liquid extraction. Although the primary goal was qualitative analysis of NAMEs (e.g., group-type separation) in produced water, the quantitative performance was also evaluated for future investigations. The instrumental detection and quantification limits were 0.10 ng mL-1 and 0.16 ng mL-1, respectively, using full spectrum data acquisition. The accuracy and precision of the proposed method ranged from 90.4 to 96.6 % and 3.3 to 13.1 %, respectively, using matrix-matched working solutions (0.1, 0.5, and 1.0 µg mL-1). The monoisotopic masses of the adduct ions ([M+H]+) and its corresponding fine isotopic patterns were used to determine the elemental composition of the NAMEs in the PW samples. Qualitative analysis indicated the O2 class as the predominant class in all samples with carbon numbers ranging from C5 to C19 and double bond equivalent (DBE) values of 1 to 8. Additional classes of polar compounds, i.e., O3, O4 and nitrogen-containing classes, are reported for the first time by gas chromatography coupled to Fourier transform Orbitrap mass spectrometry and chemical ionization.
Keywords: Derivatization; Design of experiments; Green analytical chemistry; Petroleomics; Sample preparation.
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