CRISPR/Cas12a-mediated liposome-amplified strategy for the photoelectrochemical detection of nucleic acid

Chem Commun (Camb). 2021 Sep 6;57(71):8977-8980. doi: 10.1039/d1cc03743a.

Abstract

This study reports a photoelectrochemical biosensor for dopamine-loaded liposome-encoded magnetic beads cleaved by clustered regularly interspaced short palindromic repeats (CRISPR)/Cas 12a system for the quantification of human papilloma virus (HPV)-related DNA using neodymium-doped BiOBr nanosheets (Nd-BiOBr) as a photoactive matrix. Magnetic beads and dopamine-loaded liposomes are covalently attached to the both ends of ssDNA to construct dumbbell-shaped dopamine-loaded liposome-encoded magnetic bead (DLL-MB) probes. When the guide RNA binds to the target HPV-16, the ssDNA will be cleaved by Cas12a, thereby degrading the double dumbbell probes. After magnetic separation, the dissolved DLLs are treated with Triton X-100 to release the dopamine (as an electron donor), which was then detected by an amplified photocurrent using the Nd-BiOBr-based photoelectrode.

MeSH terms

  • Bacterial Proteins / chemistry*
  • Biosensing Techniques / methods*
  • CRISPR-Associated Proteins / chemistry*
  • CRISPR-Cas Systems*
  • DNA, Single-Stranded / chemistry
  • DNA, Viral / analysis*
  • Dopamine / chemistry
  • Electrochemical Techniques / methods
  • Endodeoxyribonucleases / chemistry*
  • Fluoresceins / chemistry
  • Fluorescent Dyes / chemistry
  • Human papillomavirus 16 / chemistry
  • Liposomes / chemistry
  • Magnetic Phenomena
  • Photochemical Processes

Substances

  • Bacterial Proteins
  • CRISPR-Associated Proteins
  • DNA, Single-Stranded
  • DNA, Viral
  • Fluoresceins
  • Fluorescent Dyes
  • Liposomes
  • Cas12a protein
  • Endodeoxyribonucleases
  • Dopamine