MicroRNA-26b-5p suppresses the proliferation of tongue squamous cell carcinoma via targeting proline rich 11 (PRR11)

Liang Yi; Ying Liu; Anji Xu; Sha Li; Hailin Zhang; Mingjing Peng; Zan Li; Huayi Ren; Jie Dai; Chenhui Luo; Yazhou Xiao; Xiao Zhou; Ying Long

doi:10.1080/21655979.2021.1969832

MicroRNA-26b-5p suppresses the proliferation of tongue squamous cell carcinoma via targeting proline rich 11 (PRR11)

Bioengineered. 2021 Dec;12(1):5830-5838. doi: 10.1080/21655979.2021.1969832.

Authors

Liang Yi^{1

2

3}, Ying Liu⁴, Anji Xu^{2

3}, Sha Li^{2

3}, Hailin Zhang^{2

3}, Mingjing Peng^{1

3}, Zan Li^{2

3}, Huayi Ren¹, Jie Dai^{2

3}, Chenhui Luo³, Yazhou Xiao³, Xiao Zhou^{1

2

3}, Ying Long^{1

3}

Affiliations

¹ Translational Medicine Centre.
² Department of Head & Neck Surgery.
³ Hunan Provincial Clinical Research Centre for Oncoplastic Surgery, Hunan Cancer Hospital and the Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, Hunan, P. R. China.
⁴ Hunan Traditional Chinese Medical College, Zhuzhou, Hunan, P. R. China.

Abstract

MicroRNAs (miRNAs) have been proved to be involved in many biological processes during tumorigenesis and progression, including cell proliferation and cell cycle progression. However, the potential role of miR-26b-5p in tongue squamous cell carcinoma (TSCC) remains unclear. In the present study, we demonstrated that miR-26b-5p was decreased in TSCC tissues in both TCGA-TSCC subset and eight paired samples from TSCC patients, while Proline Rich 11 (PRR11) was obviously increased. Transfection of miR-26b-5p mimics inhibited CALL7 cell proliferation by arresting the cells at the S/G2 transition. Meanwhile, miR-26b-5p inhibitor had the opposite biological functions. The results of luciferase activity and RNA-pulldown assays indicated that miR-26b-5p directly targeted the PRR11 3' -untranslated region in CAL27 cells. Furthermore, the effects of miR-26b-5p on cell cycle regulation were reversed after treatment with siRNA against PRR11. In summary, our findings indicate that miR-26b-5p induce cell cycle arrest in TSCC by targeting PRR11. Hence, targeting miR-26b-5p could be a promising therapeutic strategy for the treatment of TSCC.

Keywords: Mir-26b-5p; cell cycle; proliferation; prr11; tongue squamous cell carcinoma.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Line, Tumor
Cell Proliferation / genetics
Humans
MicroRNAs / genetics*
MicroRNAs / metabolism
Prognosis
Proteins / genetics*
Proteins / metabolism
Squamous Cell Carcinoma of Head and Neck* / genetics
Squamous Cell Carcinoma of Head and Neck* / metabolism
Squamous Cell Carcinoma of Head and Neck* / mortality
Squamous Cell Carcinoma of Head and Neck* / pathology
Tongue / metabolism
Tongue / pathology
Tongue Neoplasms* / genetics
Tongue Neoplasms* / metabolism
Tongue Neoplasms* / mortality
Tongue Neoplasms* / pathology

Substances

MIRN26A microRNA, human
MicroRNAs
PRR11 protein, human
Proteins

Grants and funding

The present study was supported in part by the National Natural Science Foundation of China (grant no. 81703005), the Natural Science Foundation of Hunan Province (grant nos. 2017JJ3195, 2018JJ3317 and 2021JJ30427), the Hunan Provincial Science and Technology Department (grant nos. 2018SK2120, 2018SK2122, 2018SK50907 and 2018SK7005), the Research Project of Health and Family Planning Commission of Hunan Province (grant no. B2013-097, B2019092, C2019080, 20201650, 20201653 and 202102082037), Changsha Science and Technology Bureau Foundation (grant no. kq1706044 and kq1901074) and the Key Clinical Specialty Construction Project (Head&Neck Surgery) of the Provincial Health Commission of Hunan Province.