The effect of antisecretory factor on the permeability of nerve cell membrane to chloride ion

Pflugers Arch. 1987 Dec;410(6):648-51. doi: 10.1007/BF00581326.


The antisecretory factor (ASF) is a hormone-like protein (m.w. 60,000) that most effectively counteracts hypersecretion in vivo in the small intestine of pigs and rats. The present report demonstrate that 10(-13) moles of ASF inhibits significantly the 36Cl- permeation through the isolated neuronal plasma membrane of Deiters' cells in rabbits. This effect was enhanced by 0.2 mM gamma-aminobutyric acid (GABA), and quenched by the addition of anti-ASF immunoglobulins; pretreatment of the neuronal membrane with nipecotic acid (10(-6) M) or with bicuculline (10(-3) M) abolished the ASF action whilst picrotoxin (10(-4) M) pretreatment left the inhibitory effect of ASF unaffected. The results suggest that ASF blocks chloride channels in neuronal membranes, including those channels activated by GABA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antidiarrheals / pharmacology*
  • Cell Membrane Permeability / drug effects*
  • Chlorides / pharmacokinetics*
  • In Vitro Techniques
  • Neuroglia / drug effects*
  • Neuroglia / metabolism
  • Neuropeptides / pharmacology*
  • Rabbits


  • Antidiarrheals
  • Chlorides
  • Neuropeptides
  • antisecretory factor