Using Biotinylated myo-Inositol Hexakisphosphate to Investigate Inositol Pyrophosphate-Protein Interactions with Surface-Based Biosensors

Biochemistry. 2021 Sep 21;60(37):2739-2748. doi: 10.1021/acs.biochem.1c00497. Epub 2021 Sep 9.


Inositol pyrophosphates (PP-InsPs) are highly phosphorylated molecules that have emerged as central nutrient messengers in eukaryotic organisms. They can bind to structurally diverse target proteins to regulate biological functions, such as protein-protein interactions. PP-InsPs are strongly negatively charged and interact with highly basic surface patches in proteins, making their quantitative biochemical analysis challenging. Here, we present the synthesis of biotinylated myo-inositol hexakisphosphates and their application in surface plasmon resonance and grating-coupled interferometry assays, to enable the rapid identification, validation, and kinetic characterization of InsP- and PP-InsP-protein interactions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biosensing Techniques
  • Biotin / chemistry
  • Biotinylation / methods
  • Diphosphates / metabolism
  • Inositol Phosphates / chemistry*
  • Inositol Phosphates / metabolism
  • Phosphorylation
  • Phosphotransferases (Phosphate Group Acceptor) / chemistry
  • Phytic Acid / chemistry*
  • Protein Interaction Mapping / methods*
  • Signal Transduction / physiology


  • Diphosphates
  • Inositol Phosphates
  • diphosphoric acid
  • Biotin
  • Phytic Acid
  • Phosphotransferases (Phosphate Group Acceptor)