Objective: To investigate the genetic polymorphisms of Plasmodium falciparum multidrug resistance protein 1 (PfMDR1), chloroquine resistance transporter (PfCRT) and Kelch 13 (PfK13) genes in Bioko Island, Equatorial Guinea, so as to provide insights into the development of the malaria control strategy in local areas.
Methods: A total of 85 peripheral blood samples were collected from patients with Plasmodium falciparum infections in Bioko Island, Equatorial Guinea in 2018 and 2019, and genomic DNA was extracted. The PfMDR1, PfCRT and PfK13 genes were amplified using a nested PCR assay. The amplification products were sequenced, and the gene sequences were aligned.
Results: There were no mutations associated with artemisinin resistance in PfK13 gene in Bioko Island, Equatorial Guinea, while drug-resistant mutations were detected in PfMDR1 and PfCRT genes, and the proportions of PfMDR1_N86Y, PfMDR1_Y184F and PfCRT_K76T mutations were 35.29% (30/85), 72.94% (62/85) and 24.71% (21/85), respectively.
Conclusions: There are mutations in PfMDR1, PfCRT and PfK13 genes in P. falciparum isolates from Bioko Island, Equatorial Guinea.
［摘要］ 目的 调查赤道几内亚Bioko岛恶性疟原虫多药耐药蛋白1 (Plasmodium falciparum multidrug resistance protein1, PfMDR1)、氯喹抗性转运蛋白基因 (P. falciparum chloroquine resistant transporter, PfCRT) 和Kelch 13 基因 (P. falciparum Kelch 13, PfK13) 多态性, 为当地疟疾防控策略制定提供参考。方法 采集2018—2019年赤道几内亚Bioko岛恶性疟原虫感染者外周血样本85份, 提取基因组DNA。采用巢式PCR技术扩增PfMDR1、PfCRT 和PfK13 基因, 对扩增产物进行DNA测序, 并对基因序列进行比对。结果 赤道几内亚Bioko岛恶性疟原虫PfK13 基因不存在已知与青蒿素抗性相关的突变; PfMDR1 基因和PfCRT 基因均存在不同比例抗药性突变, 其中PfMDR1_N86Y、PfMDR1_Y184F和PfCRT_K76T突变率分别为35.29% (30/85)、72.94% (62/85) 和24.71% (21/85)。结论 赤道几内亚Bioko岛恶性疟原虫PfMDR1、PfCRT 基因和PfK13 基因均存在不同程度突变。.
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