A Tad-like apparatus is required for contact-dependent prey killing in predatory social bacteria

Elife. 2021 Sep 10;10:e72409. doi: 10.7554/eLife.72409.

Abstract

Myxococcus xanthus, a soil bacterium, predates collectively using motility to invade prey colonies. Prey lysis is mostly thought to rely on secreted factors, cocktails of antibiotics and enzymes, and direct contact with Myxococcus cells. In this study, we show that on surfaces the coupling of A-motility and contact-dependent killing is the central predatory mechanism driving effective prey colony invasion and consumption. At the molecular level, contact-dependent killing involves a newly discovered type IV filament-like machinery (Kil) that both promotes motility arrest and prey cell plasmolysis. In this process, Kil proteins assemble at the predator-prey contact site, suggesting that they allow tight contact with prey cells for their intoxication. Kil-like systems form a new class of Tad-like machineries in predatory bacteria, suggesting a conserved function in predator-prey interactions. This study further reveals a novel cell-cell interaction function for bacterial pili-like assemblages.

Keywords: Myxococcus xanthus; infectious disease; microbiology; motility; predation; tad pilus.

Publication types

  • Research Support, Non-U.S. Gov't
  • Video-Audio Media

MeSH terms

  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism*
  • Escherichia coli / growth & development*
  • Fimbriae, Bacterial / genetics
  • Fimbriae, Bacterial / metabolism*
  • Microbial Viability
  • Movement
  • Myxococcus xanthus / genetics
  • Myxococcus xanthus / metabolism*
  • Myxococcus xanthus / pathogenicity
  • Single-Cell Analysis
  • Soil Microbiology*
  • Time Factors

Substances

  • Bacterial Proteins

Associated data

  • SRA/PRJNA408275

Grant support

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.