Impact of bisphenol-A and synthetic estradiol on brain, behavior, gonads and sex hormones in a sexually labile coral reef fish
- PMID: 34507054
- DOI: 10.1016/j.yhbeh.2021.105043
Impact of bisphenol-A and synthetic estradiol on brain, behavior, gonads and sex hormones in a sexually labile coral reef fish
Abstract
Endocrine disrupting chemicals, such as bisphenol A (BPA) and ethinylestradiol (EE2), are detected in the marine environment from plastic waste and wastewater effluent. However, their impact on reproduction in sexually labile coral reef fish is unknown. The objective of this study was to determine impacts of environmentally relevant concentrations of BPA and EE2 on behavior, brain gene expression, gonadal histology, sex hormone profile, and plasma vitellogenin (Vtg) levels in the anemonefish, Amphiprion ocellaris. A. ocellaris display post-maturational sex change from male to female in nature. Sexually immature, male fish were paired together and fed twice daily with normal food (control), food containing BPA (100 μg/kg), or EE2 (0.02 μg/kg) (n = 9 pairs/group). Aggression toward an intruder male was measured at 1, 3, and 6 months. Blood was collected at 3 and 6 months to measure estradiol (E2), 11-ketotestosterone (11-KT), and Vtg. At the end of the study, fish were euthanized to assess gonad morphology and to measure expression of known sexually dimorphic genes in the brain. Relative to control, BPA decreased aggression, altered brain transcript levels, increased non-vitellogenic and vitellogenic eggs in the gonad, reduced 11-KT, and increased plasma Vtg. In two BPA-treated pairs, both individuals had vitellogenic eggs, which does not naturally occur. EE2 reduced 11-KT in subordinate individuals and altered expression of one transcript in the brain toward the female profile. Results suggest BPA, and to a lesser extent EE2, pollution in coral reef ecosystems could interfere with normal reproductive physiology and behavior of the iconic sexually labile anemonefish.
Keywords: 11-Ketotestosterone; Aggression; Bisphenol A; EDC; Endocrine disruptor; Estradiol; Ethinylestradiol; Feminization; Gonad; Plastic; Pollution; Protandrous sex change; Reproduction; Vitellogenic; Xenoestrogen.
Copyright © 2021 Elsevier Inc. All rights reserved.
Similar articles
-
Effects of Dietary Bisphenol A on the Reproductive Function of Gilthead Sea Bream (Sparus aurata) Testes.Int J Mol Sci. 2019 Oct 10;20(20):5003. doi: 10.3390/ijms20205003. Int J Mol Sci. 2019. PMID: 31658598 Free PMC article.
-
Fighting Nemo: Effect of 17α-ethinylestradiol (EE2) on aggressive behavior and social hierarchy of the false clown anemonefish Amphiprion ocellaris.Mar Pollut Bull. 2017 Nov 30;124(2):760-766. doi: 10.1016/j.marpolbul.2016.12.042. Epub 2016 Dec 27. Mar Pollut Bull. 2017. PMID: 28034494
-
Effects of BPA on zebrafish gonads: Focus on the endocannabinoid system.Environ Pollut. 2020 Sep;264:114710. doi: 10.1016/j.envpol.2020.114710. Epub 2020 May 1. Environ Pollut. 2020. PMID: 32417572
-
[Metamorphosis of marine fish larvae and thyroid hormones].Biol Aujourdhui. 2019;213(1-2):27-33. doi: 10.1051/jbio/2019010. Epub 2019 Jul 5. Biol Aujourdhui. 2019. PMID: 31274100 Review. French.
-
Sex change in the protandrous black porgy, Acanthopagrus schlegeli: a review in gonadal development, estradiol, estrogen receptor, aromatase activity and gonadotropin.J Exp Zool. 2001 Dec 1;290(7):715-26. doi: 10.1002/jez.1122. J Exp Zool. 2001. PMID: 11748620 Review.
Cited by
-
Independent and synergistic effects of microplastics and endocrine-disrupting chemicals on the reproductive social behavior of fathead minnows (Pimephales promelas).Ecol Evol. 2024 Feb 6;14(2):e10846. doi: 10.1002/ece3.10846. eCollection 2024 Feb. Ecol Evol. 2024. PMID: 38327688 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
