High Expression of ROMO1 Aggravates the Malignancy of Hepatoblastoma

Jiangfeng Lv; Yan Wu; Wei Li; Huaping Fan

doi:10.1155/2021/2341719

High Expression of ROMO1 Aggravates the Malignancy of Hepatoblastoma

J Oncol. 2021 Sep 1:2021:2341719. doi: 10.1155/2021/2341719. eCollection 2021.

Authors

Jiangfeng Lv¹, Yan Wu², Wei Li³, Huaping Fan⁴

Affiliations

¹ Department of Clinical Laboratory, Jinan City People's Hospital, Jinan People's Hospital Affiliated to Shandong First Medical University, Jinan, Shandong 271199, China.
² Department of Clinical Laboratory, Yantai Yuhuagding Hospital Affiliated to Qingdao University, Yantai, Shandong 264000, China.
³ Department of Oncology (II), Qingdao Central Hospital Affiliated to Qingdao University, Qingdao, Shandong 266042, China.
⁴ Department of Pediatrics, Yantai Maternal and Child Health Care Hospital, Yantaishan Hospital, Yantai, Shandong 264000, China.

Abstract

Hepatoblastoma (HB) is a kind of tumor that occurs frequently in children and is highly malignant. Here, the function of ROS modulator 1 (ROMO1) was identified in the development of HB. In this study, the mRNA expression of ROMO1 was measured by RT-qPCR. Colony formation assay, MTT assay, and flow cytometric analysis were applied to detect cell viability. The cell migrative and invasive ability was measured by wound healing and transwell assays. Tumor xenografts were performed to examine tumor growth. The results showed that upregulation of ROMO1 was identified in liver hepatocellular carcinoma (LIHC) tissues and predicted poor prognosis in LIHC patients. And ROMO1 expression was also increased in HB tissues and cells. Functionally, ROMO1 knockdown restrained cell viability, migration, and invasion in HB. In addition, knockdown of ROMO1 was found to suppress tumor formation in vivo. In conclusion, upregulation of ROMO1 promotes tumor growth and cell aggressiveness in HB.