Trans-Serosal Multimodal Optical Coherence Tomography for Visualization of Microstructure and Blood Circulation of the Small Intestine Wall

M G Ryabkov; E B Kiseleva; M S Baleev; E L Bederina; M A Sizov; A N Vorobyov; A A Moiseev; M M Karabut; M A Plekhanova; N D Gladkova

doi:10.17691/stm2020.12.2.07

Trans-Serosal Multimodal Optical Coherence Tomography for Visualization of Microstructure and Blood Circulation of the Small Intestine Wall

Sovrem Tekhnologii Med. 2020;12(2):56-64. doi: 10.17691/stm2020.12.2.07.

Authors

M G Ryabkov¹, E B Kiseleva², M S Baleev³, E L Bederina⁴, M A Sizov³, A N Vorobyov³, A A Moiseev⁵, M M Karabut⁶, M A Plekhanova⁷, N D Gladkova⁸

Affiliations

¹ Associate Professor, Leading Researcher, University Clinic, Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Square, Nizhny Novgorod, 603005, Russia.
² Senior Researcher, Scientific Laboratory of Optical Coherence Tomography, Institute of Experimental Oncology and Biomedical Technologies, Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Square, Nizhny Novgorod, 603005, Russia.
³ Surgeon, City Clinical Hospital No.30, 85A Berezovskaya St., Nizhny Novgorod, 605157, Russia.
⁴ Pathologist, Junior Researcher, University Clinic, Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Square, Nizhny Novgorod, 603005, Russia.
⁵ Senior Researcher, Laboratory of Highly Sensitive Optical Measurements, Federal Research Center Institute of Applied Physics of the Russian Academy of Sciences, 46 Ul'yanova St., Nizhny Novgorod, 603950, Russia.
⁶ Researcher, Genomics Adaptive Antitumor Immunity Research Laboratory, Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Square, Nizhny Novgorod, 603005, Russia.
⁷ Student, Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Square, Nizhny Novgorod, 603005, Russia.
⁸ Professor, Head of the Scientific Laboratory of Optical Coherence Tomography, Institute of Experimental Oncology and Biomedical Technologies, Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Square, Nizhny Novgorod, 603005, Russia.

Abstract

The aim of the study was to evaluate the performance of trans-serosal multimodal OCT (MM OCT) in in vivo detecting of changes in microstructure and blood circulation of the small intestine wall caused by arteriovenous ischemia resulted from intestine strangulation.

Materials and methods: In experiments on Wistar rats (n=22), we examined the small intestine wall in vivo using MM OCT; the access to the intestine was reached through laparotomy. The microvasculature and microstructure of the wall were studied before and after acute arteriovenous ischemia created by ligation of a small bowel segment. The results were then added with data obtained from histological and intravital microscopic examination.

Results: Trans-serous MM OCT allowed us to visualize the bowel wall to its entire thickness, distinguish between the serous-muscular and mucous-submucosal layers, and detect the villi and functioning blood vessels. The structures were best seen after a fat emulsion had been administered into the bowel lumen. In OCT images made in the optical coherent angiography (OCA) mode, large paired vessels (arteries and veins) and micro-vessels with a diameter of >15 μm could be seen. Most of the blood vessels were imaged in the depth range of 80-300 μm from the surface. Capillaries with a diameter of 7-10 μm were not seen, but they produced an overall bright background. In the OCA images reconstructed from a volume of 2.4×2.4×1.8 mm, the total length of the vascular bed before ischemia was 18.3 [16.6; 19.8] mm.Strangulation of the intestinal loop was associated with changes in the CP OCT picture: the villi-associated vertical pattern and shadows of blood vessels disappeared and the depth of tissue visualization in the cross-channel decreased. The optical equivalents of the serous-muscular layer were preserved; after 180±12 min of ischemia, their proportion in the intestinal wall thickness increased from 25 [18; 32] to 42 [31; 55]% (p=0.031). At that time-point, OCA images of the strangulated bowel loop looked all similar: a uniform dark background with isolated fragmentary large vessels and no signs of blood flow in the microvascular network.

Conclusion: Trans-serous MM OCT provides for in vivo visualization of microstructures critical for surgical gastroenterology: the intestinal wall layers including villi and blood vessels of each layer, as confirmed by histological analysis. Destructive processes in the intestinal wall resulting from bowel ligation bring about optical changes, which can be detected using real-time MM OCT.

Keywords: CP OCT; cross-polarization optical coherence tomography; histomorphometry; optical coherence angiography; small bowel ischemia; small intestinal strangulation; viability of the small intestine.