This study was performed to evaluate the effects of different amounts and particle size of zinc oxide (ZnO) on villus height (VH), villus width (VW), crypt depth (CD) and VH to CD ratio (VH: CD), and expression of zonula occludens-1 (ZO-1), occludin (OC) and tumour necrosis factor-α (TNF-α) in broiler breeders. A total of 350 (Ross 308) broiler breeder hens of 54 weeks randomly assigned to seven treatments, included control basal diet (C) without added Zn, C+ 100, and 130 mg Zn per kg of diet from Large (L) (100-1000 nm) and Small (S) (<100 nm) particle size ZnO (LZnO100 and 130; SZnO100 and 130), C and SZnO100 challenged with lipopolysaccharide (C+LPS and SZnO100+LPS). Each diet was fed to five replicates consisting of ten birds each. The middle part of the duodenum, jejunum and ileum was used for morphological assessments. To assess the gene expression of ZO-1, OC and TNF-α in the jejunum samples were excised. Results showed that the supplementing 130 ppm SZnO increased VH:CD in the duodenum (p < 0.05). VW in the duodenum and all the evaluated morphometric indices in jejunum and ileum were not affected by the dietary treatment (p > 0.05). ZO-1 mRNA abundance in C+LPS group compared to SZnO100+LPS group was significantly decreased and increased by LPS and SZnO100 respectively. The SZnO-100 increased OC gene expression in compare to C+LPS group. The expression of TNF-α in C+LPS treatment was higher than other groups (p < 0.05). The lowest and the highest litter moisture and foot-pad dermatitis (FPD) were observed in LZnO-130 and C treatments respectively (p < 0.05). Improving the physical properties of ZnO affect on VH:CD. Broiler breeder diet with ZnO enhance ZO-1, OC and mitigate TNF-α gene expression in jejunum maintenance of gut health in broiler breeders.
Keywords: foot-pad dermatitis; gene expression; gut health; inflammation; intestinal morphology.
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