Analysis of codon-specific translation by ribosome profiling

Methods Enzymol. 2021;658:191-223. doi: 10.1016/bs.mie.2021.06.025. Epub 2021 Aug 23.


Chemical modifications of RNA molecules can affect translation in multiple ways. Therefore, it is critical to understand how their absence changes cellular translation dynamics and in particular codon-specific translation. In this chapter, we discuss the application of ribosome profiling to analyze changes in codon-specific translation and differential translation in Saccharomyces cerevisiae and human cells.

Keywords: Codon-specific translation; RNA modification; Ribosome profiling; tRNA.

MeSH terms

  • Codon / genetics
  • Codon / metabolism
  • Humans
  • Protein Biosynthesis*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • RNA, Transfer* / genetics
  • RNA, Transfer* / metabolism
  • Ribosomes / genetics
  • Ribosomes / metabolism


  • Codon
  • RNA, Messenger
  • RNA, Transfer