bFGF-mediated phosphorylation of δ-catenin increases its protein stability and the ability to induce the nuclear redistribution of β-catenin

Gen Chen; Ning An; Yu Zhu; Rui Zhou; Myung-Giun Noh; Hangun Kim; Hyoung Jae Lee; Yingjie Shen; Young-Chang Cho; Litai Jin; Weitao Cong; Jae-Hyuk Lee; Kwonseop Kim

bFGF-mediated phosphorylation of δ-catenin increases its protein stability and the ability to induce the nuclear redistribution of β-catenin

Am J Cancer Res. 2021 Aug 15;11(8):3877-3892. eCollection 2021.

Authors

Gen Chen^{1

2}, Ning An³, Yu Zhu², Rui Zhou⁴, Myung-Giun Noh⁵, Hangun Kim⁴, Hyoung Jae Lee¹, Yingjie Shen^{1

2}, Young-Chang Cho¹, Litai Jin², Weitao Cong², Jae-Hyuk Lee⁶, Kwonseop Kim¹

Affiliations

¹ College of Pharmacy, Chonnam National University Gwangju 61186, Korea.
² School of Pharmaceutical Science, Wenzhou Medical University Wenzhou 325000, P. R. China.
³ Department of Pharmacy, Ningbo Medical Center Lihuili Hospital Ningbo 315041, P. R. China.
⁴ College of Pharmacy, Sunchon National University Sunchon 57922, Korea.
⁵ Department of Biomedical Science and Engineering, Gwangju Institute of Science and Technology (GIST) Gwangju 61005, Korea.
⁶ Chonnam National University Hwasun Hospital & Medical School Hwasun 58128, Korea.

PMID: 34522455
PMCID: PMC8414378

Abstract

Recently, we have shown that δ-catenin strengthened the epidermal growth factor receptor (EGFR)/Erk1/2 signaling pathway through the association between EGFR and δ-catenin. Now, we further analyzed the correlation between basic fibroblast growth factor (bFGF)/fibroblast growth factor receptor 1 (FGFR1) and δ-catenin in prostate cancer and investigated the molecular mechanism underlying the role of bFGF/FGFR1 modulation in CWR22Rv-1 (Rv-1) cells. Here, we demonstrated that bFGF phosphorylated the tyrosine residues of δ-catenin in Rv-1 cells and further proved that the bFGF mediated FGFR1/δ-catenin tyrosine phosphorylation was time dependent. Furthermore, we demonstrated that bFGF stabilized the expression of δ-catenin through weakening its association with GSK3β and enhancing its stability to induce β-catenin into the nuclear by strengthening the processing of E-cadherin. In a word, these results indicated that bFGF/FGFR1 signaling pathway could enhance the tumor progression of prostate cancer via δ-catenin.

Keywords: E-cadherin; FGFR1; GSK3β; bFGF; δ-catenin.