Deuteration of terminal alkynes realizes simultaneous live cell Raman imaging of similar alkyne-tagged biomolecules

Syusuke Egoshi; Kosuke Dodo; Kenji Ohgane; Mikiko Sodeoka

doi:10.1039/d1ob01479j

Deuteration of terminal alkynes realizes simultaneous live cell Raman imaging of similar alkyne-tagged biomolecules

Org Biomol Chem. 2021 Oct 6;19(38):8232-8236. doi: 10.1039/d1ob01479j.

Authors

Syusuke Egoshi¹, Kosuke Dodo^{1

2}, Kenji Ohgane¹, Mikiko Sodeoka^{1

2}

Affiliations

¹ Synthetic Organic Chemistry Laboratory, RIKEN Cluster for Pioneering Research, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan. dodo@riken.jp.
² RIKEN Center for Sustainable Resource Science, 2-1, Hirosawa, Wako, Saitama 351-0198, Japan.

PMID: 34528645
DOI: 10.1039/d1ob01479j

Abstract

Alkynes were employed as tags to observe small molecules in cells by Raman microscopy. Herein, simple deuteration was found to shift the vibrational frequency of the alkyne by 135 cm^-1. Two-color Raman imaging of D-alkynes and H-alkynes made it possible to distinguish between and observe similar small molecules in live cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alkynes*

Substances

Alkynes