The Cellular Mechanisms of Dopamine Modulation on the Neuronal Network Oscillations in the CA3 Area of Rat Hippocampal Slices

Xin'e Xie; Mingcan Li; Bingyan Feng; Junmei Li; Zhongyu Sun; Ying Zhao; Chengbiao Lu

doi:10.1016/j.neuroscience.2021.09.005

The Cellular Mechanisms of Dopamine Modulation on the Neuronal Network Oscillations in the CA3 Area of Rat Hippocampal Slices

Neuroscience. 2021 Nov 1:475:83-92. doi: 10.1016/j.neuroscience.2021.09.005. Epub 2021 Sep 14.

Authors

Xin'e Xie¹, Mingcan Li², Bingyan Feng³, Junmei Li³, Zhongyu Sun³, Ying Zhao⁴, Chengbiao Lu⁵

Affiliations

¹ Henan International-Joint Laboratory for Non-invasive Neural Modulation/The Key Laboratory for the Brain Research of Henan Province, Department of Physiology, Xinxiang Medical University, Xinxiang 453003, China; The Second Hospital of Jinhua, Jinhua, Zhejiang 321000, China.
² Henan International-Joint Laboratory for Non-invasive Neural Modulation/The Key Laboratory for the Brain Research of Henan Province, Department of Physiology, Xinxiang Medical University, Xinxiang 453003, China; Key Laboratory of Clinical Psychopharmacology, School of Pharmacy, Xinxiang Medical University, Xinxiang 453003, China.
³ Henan International-Joint Laboratory for Non-invasive Neural Modulation/The Key Laboratory for the Brain Research of Henan Province, Department of Physiology, Xinxiang Medical University, Xinxiang 453003, China.
⁴ Key Laboratory of Clinical Psychopharmacology, School of Pharmacy, Xinxiang Medical University, Xinxiang 453003, China.
⁵ Henan International-Joint Laboratory for Non-invasive Neural Modulation/The Key Laboratory for the Brain Research of Henan Province, Department of Physiology, Xinxiang Medical University, Xinxiang 453003, China. Electronic address: johnlu9000@163.com.

PMID: 34534635
DOI: 10.1016/j.neuroscience.2021.09.005

Abstract

Network oscillations at γ frequency band (30-80 Hz), generated by the interaction between inhibitory interneurons and excitatory neurons, have been proposed to be associated with higher brain functions such as learning and memory. Dopamine (DA), one of the major CNS transmitters, modulates hippocampal γ oscillations but the intracellular mechanisms involved remain elusive. In this study, we recorded kainate-induced γ oscillations in the CA3 area of rat hippocampal slices, and found that DA strongly enhanced γ power, which was largely blocked by dopamine receptor 1 (DR1) antagonist SCH23390, receptor tyrosine kinase (RTK) inhibitor UNC569 and ERK inhibitor U0126, partially blocked by D2/3R antagonist raclopride, PKA inhibitor H89 and PI3K inhibitor wortmannin, but not affected by AKT inhibitor TCBN or NMDAR antagonist D-AP5. Our results indicate that DA-mediated γ enhancement is involved in the activation of signaling pathway of DR1/2-RTK-ERK. Our data demonstrate a strong, rapid modulation of DA on hippocampal γ oscillations and provide a new insight into cellular mechanisms of DA-mediated γ oscillations.

Keywords: ERK; PKA; RTK; dopamine; γ oscillations.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Dopamine*
Hippocampus
Kainic Acid
Neurons
Phosphatidylinositol 3-Kinases*
Rats

Substances

Kainic Acid
Dopamine