miR-497-5p induces apoptosis in K562 cells by downregulating ROCK1

Nafei Chen; Zhen Meng; Jiaojie Song; Lingfang Kong; Yehua Zhang; Suli Guo; Xiaokun Zhang; Xin Lu; Licai Jiang; Ran Chen; Zongjiu Jiao; Liyun Zhao

miR-497-5p induces apoptosis in K562 cells by downregulating ROCK1

Am J Transl Res. 2021 Aug 15;13(8):9278-9284. eCollection 2021.

Authors

Nafei Chen¹, Zhen Meng², Jiaojie Song¹, Lingfang Kong¹, Yehua Zhang¹, Suli Guo¹, Xiaokun Zhang¹, Xin Lu¹, Licai Jiang¹, Ran Chen¹, Zongjiu Jiao¹, Liyun Zhao¹

Affiliations

¹ Department of Hematology, Xingtai People's Hospital Xingtai 054000, Hebei Province, China.
² Department of Hematology, Hudson International Peace Hospital, Heng Shui City People's Hospital Hengshui 053000, Hebei Province, China.

PMID: 34540044
PMCID: PMC8430193

Abstract

Objective: To validate the role of miR-497-5p in apoptosis in K562 cells by targeting Rho-associated kinase isoform 1 (ROCK1).

Methods: From January, 2017 to February, 2019, 57 patients with chronic myeloid leukemia (CML) treated in our hospital were included in patient group, and 50 healthy individuals were recruited as control group. miR-497-5p level in peripheral blood was quantitated using qRT-PCR. After transfecting with miR-497-5p overexpression vector and ROCK1 inhibitor, K562 cells were monitored in terms of proliferation (CCK8 assay), migration and invasion (Transwell), and apoptosis (flow cytometry). Binding loci between miR-497-5p and ROCK1 were predicted, and the targeting relationship was confirmed (dual-luciferase reporter (DLR) assay).

Results: miR-497-5p was poorly expressed in CML (P < 0.05). Forced overexpression of miR-497-5p or inhibition of ROCK1 suppressed malignant processes (proliferation, proliferation, migration and invasion) in K562 cells and induced apoptosis (P < 0.05). DLR assay revealed a decreased luciferase activity after miR-497-5p binding to ROCK1 (P < 0.05).

Conclusion: miR-497-5p induces apoptosis in K562 cells by downregulating ROCK1.

Keywords: K562 cells; ROCK1; apoptosis; invasion and migration; miR-497-5p.