Uptake Assays to Monitor Anthracyclines Entry into Mammalian Cells

Nicolas Brosseau; Emil Andreev; Dindial Ramotar

doi:10.21769/BioProtoc.2555

Uptake Assays to Monitor Anthracyclines Entry into Mammalian Cells

Bio Protoc. 2017 Sep 20;7(18):e2555. doi: 10.21769/BioProtoc.2555.

Authors

Nicolas Brosseau¹, Emil Andreev¹, Dindial Ramotar¹

Affiliation

¹ Maisonneuve-Rosemont Hospital Research Center, and the Université de Montréal, Faculty of Medicine, Department of Medicine, Montréal, Quebec, Canada.

Abstract

Anthracyclines, such as doxorubicin and daunorubicin, are DNA damaging agents that autofluoresce and can be readily detected in cells. Herein, we developed suitable assays to quantify and localize daunorubicin in mammalian cells. These assays can be exploited to identify components that are involved in the uptake of anthracyclines.

Keywords: Anthracyclines; Autofluorescent drugs; Epifluorescence microscopy; FACS analysis; Fluoroskan reader; Influx transporters.