Preparation of Primary Astrocyte Culture Derived from Human Glioblastoma Multiforme Specimen

Mansoureh Hashemi; Mahmoudreza Hadjighassem

doi:10.21769/BioProtoc.2241

Preparation of Primary Astrocyte Culture Derived from Human Glioblastoma Multiforme Specimen

Bio Protoc. 2017 Apr 20;7(8):e2241. doi: 10.21769/BioProtoc.2241.

Authors

Mansoureh Hashemi¹, Mahmoudreza Hadjighassem^{2

3}

Affiliations

¹ Functional Neurosurgery Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
² Department of Neuroscience, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
³ Brain and Spinal Cord Injury Research Center, Neuroscience Institute, Tehran University of Medical Sciences, Tehran, Iran.

Abstract

Glioblastoma multiforme (GBM) is a grade 4 astrocytoma tumor in central nervous system. Astrocytes can be isolated from human GBM. Study of astrocytes can provide insights about the formation, progression and recurrence of glioblastoma. For using isolated astrocytes, new studies can be designed in the fields of pharmacology, neuroscience and neurosurgery for glioblastoma treatment. This protocol describes the details for preparing high purity primary astrocytes from human GBM. Tumor tissue is disrupted using mechanical dissociation and chemical digestion in this protocol. 2 weeks after plating the cell suspension in culture, primary astrocytes are available for further subculturing and immunocytochemistry of S100-beta antigen.

Keywords: Astrocyte; Astrocytoma tumor; Glioblastoma multiforme; Malignant brain tumor; Primary cell culture; S100-beta antigen.