Kombucha tea from seagrapes (Caulerpa racemosa) potential as a functional anti-ageing food: in vitro and in vivo study

Happy Kurnia Permatasari; Fahrul Nurkolis; Piko Satria Augusta; Nelly Mayulu; Mury Kuswari; Nurpudji Astuti Taslim; Defny Silvia Wewengkang; Siti Chairiyah Batubara; William Ben Gunawan

doi:10.1016/j.heliyon.2021.e07944

Kombucha tea from seagrapes (Caulerpa racemosa) potential as a functional anti-ageing food: in vitro and in vivo study

Heliyon. 2021 Sep 8;7(9):e07944. doi: 10.1016/j.heliyon.2021.e07944. eCollection 2021 Sep.

Authors

Happy Kurnia Permatasari¹, Fahrul Nurkolis², Piko Satria Augusta³, Nelly Mayulu⁴, Mury Kuswari⁵, Nurpudji Astuti Taslim⁶, Defny Silvia Wewengkang⁷, Siti Chairiyah Batubara⁸, William Ben Gunawan⁹

Affiliations

¹ Department of Biochemistry and Biomolecular, Faculty of Medicine, Brawijaya University, Malang, Indonesia.
² Biological Sciences, Faculty of Sciences and Technology, UIN Sunan Kalijaga, Yogyakarta, Indonesia.
³ Faculty of Medicine, Brawijaya University, Malang, Indonesia.
⁴ Nutrition, Faculty of Medicine, Sam Ratulangi University, Manado, Indonesia.
⁵ Nutrition, Faculty of Health Sciences, Universitas Esa Unggul, Jakarta, Indonesia.
⁶ Clinical Nutrition, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia.
⁷ Pharmacy, Faculty of Mathematics and Sciences, Sam Ratulangi University, Manado, Indonesia.
⁸ Food Technology, Faculty of Food Technology and Health, Sahid University, Jakarta, Indonesia.
⁹ Nutrition, Faculty of Medicine, Diponegoro University, Semarang, Indonesia.

Abstract

This study wants to investigate the effects of kombucha tea based on seagrapes on blood glucose levels, total cholesterol, and PGC-1α in Swiss albino mice that were given cholesterol- and fat-enriched diets (CFED). Anti-glycation, tyrosinase inhibitory, and α-glucosidase inhibitory activity were also determined. Forty male swiss webster albino mice weighing between 20 g-30 g were used for this study. Animals were distributed in random into 4 groups of 10 animals each; group A served as normal control (received standard dry pellet diet), group B were fed on CFED for 4 weeks, and groups C and D were fed on CFED and were administered 150 and 300 mg/kg of kombucha tea from seagrapes (Caulerpa racemosa) (p.o.). In vitro study show that the activity of anti-glycation, L-Tyrosine, L-Dopa, α-glucosidase, and α-amylase inhibition were 62.79 ± 0.78, 9.05 ± 0.16, 27.14 ± 1.62, 90.42 ± 0.77, and 80.44 ± 1.00, respectively. Group C has a better activity in increasing PGC-1-alpha serum in mice than group D (p < 0.05). There were no meaningful differences between group C and D in blood cholesterol and blood glucose reduction (p = 0.222), both groups have the same effect in lowering total cholesterol and blood glucose in mice. In conclusion, kombucha tea from seagrapes has potential as an anti-ageing functional food.

Keywords: Anti-ageing; Anti-glycation; Blood glucose; Caulerpa racemosa; Functional food; PGC1α; Total cholesterol; Tyrosinase inhibitory; α-glucosidase.